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This is a large and busy commercial harbour with specific areas set aside for pleasure craft.
See Tony Herrick's of Cruising Connections free e-book WELCOME TO DURBAN (A Sailor's Guide for making safe landfall in South Africa).
See South Africa Formalities, for full details on clearing into and out of South Africa.
Durban Harbour Radio (the Signal tower that controls shipping movements into and out of the harbour) must be contacted approx. 1 mile off on VHF channel 09, to inform the authorities in advance of the yacht's arrival and to request permission to proceed through the entrance channel. Foreign yachts may be met by a Police Coast Patrol which will escort the yacht to a holding area, or alternatively Durban Harbour Radio may advise to proceed to the International Jetty or Small Craft Basin.
Arriving yachts have to contact the following departments within 24 hours of arrival: Customs, Immigration, Health, and Portnet Office. These offices must also be visited when leaving. Note all International visitors must report to Durban Immigrations, irrespective of whether they have cleared into South Africa already. Durban Marina can help arrange clearance.
If leaving for another South African port, lodge an itinerary (flight plan) with the Port Authority (time of departure, intended destination, and ETA) and clearance obtained from the yacht club or marina you are in.
NOTE: The Port Authority office is NOT open on weekends and Public Holidays, therefore clearing out should be done on the Friday morning prior to leaving.
Last updated December 2012.
The harbour is approached via a narrow channel, and this protects it from prevailing NE and SW winds, both of which can blow with considerable force.
Docking is available at Durban Marina or on moorings at the smaller Bluff Yacht Club, 5nm from the harbour entrance. The Royal Natal Yacht Club and the Point Yacht Club, based at the Durban Marina, no longer have berthing facilities available but there is an anchorage in front.
It is possible to anchor in the Silburn Channel, where the holding is reasonably good. Fore and aft anchors are required and care should be taken not to obstruct the channel.
Last updated December 2012.
The Point Yacht Club and Royal Natal Yacht Club have internet facilities, as well as good bars and restaurants, which visiting yachtsmen are welcome to use when berthed at the Durban Marina. Yachtsmen have a week’s free membership with either club to use their facilities.


All forms require users to input data, these are the building blocks for basic inputs and text fields.
To use the components here you first need to include this module in your application @NgModule


--- layout: post authors: ["Greg Wilson"] title: Why We Teach date: 2013-01-04 time: "10:00:00" favorite: true tags: ["Noticed"] ---
Data Sharing and Management Snafu in 3 Short Acts: you have to laugh, because otherwise you'd cry...


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My interest in software development began back in college when I took a business programming class. I found the class challenging yet intriguing. It was too late in my degree program to transition to anything more technical, so I graduated from Seattle University with a bachelor of arts in Business Administration and an Entrepreneurship minor. Throughout college I worked in the service industry supporting myself working jobs baking, ice cream making, and in catering operations. After graduating I went on to work in hospitality as a hotel sales coordinator, catering sales manager, and event manager. Beginning to feel burnt out I decided to pursue software development. I spent almost a year attempting to self-study via online courses, attending a few women who code workshops, and taking time to travel, but then decided that more formal instruction was key to making a career transition.
When I’m not attending class at the University of Washington’s Full Stack Web Development Boot Camp or studying, I enjoy cooking, baking, traveling, playing the world’s only 10-player arcade game Killer Queen, reading, and spending time with friends and family. Upon completion of the boot camp I’m looking forward to using my software developer skills to work in the non-profit field.


 You pick out a cute dress from your wardrobe and try it on. Looking in the mirror you feel slightly off, but for the most part you feel cute and are happy with your decision overall.
The end


Our extremely accurate algorithmns have read your personality and the results are:
python is a general purpose programming language that focuses on human readability. This makes it a great language to learn as a beginner. The number of features in the language itself is modest, requiring relatively little investment of time or effort to produce your first programs. The Python syntax is designed to be readable and straightforward. This simplicity makes Python an ideal teaching language, and it lets newcomers pick it up quickly. As a result, developers spend more time thinking about the problem they’re trying to solve and less time thinking about language complexities or deciphering code left by others.
C# is a general-purpose, object-oriented programming language. It is intended for a simple, modern and general-purpose language. It has been designed to build software ranging from small functions to large operating systems. It is also a multi-paradigm language that is strong typed, imperative, declarative, functional, and component-oriented.
JavaScript is a high-level programming language. It is mostly used in web browsers. Along with HTML and CSS, JavaScript is the foundation of the world wide web. It makes the interaction between client and server possible. A vast majority of websites use JavaScript and almost all modern web browsers have a JavaScript engine. JavaScript supports event-driven, functional, and imperative programming styles, it is a multi-paradigm language. It can work with text, arrays, regular expressions, dates, and can perform basic manipulation of the document object model (DOM).
Academics and statisticians have developed R over two decades. R has now one of the richest ecosystems to perform data analysis. There are around 12000 packages available in CRAN (open-source repository). It is possible to find a library for whatever the analysis you want to perform. The rich variety of library makes R the first choice for statistical analysis, especially for specialized analytical work. The cutting-edge difference between R and the other statistical products is the output. R has fantastic tools to communicate the results.


Today is Tuesday, Oct 14, 2014
Reminder !! Admin
38% John Profile Completeness
- Menu
- Dashboard
- Spring Admin
- Core HR
- HR Reports
- Employees
- Self Service
- Performance Management
- Talent Management
- Time & Attendance
- Leave Management
- Benefits Administration
- Schedule Management
- Payroll USA
- Training & Development
- Spring Extras
Spring Solutions Inc
© Copyrights 2014


My mission is to become a full stack web developer at a large cap tech company somewhere on the West Coast. Former #1 rated old-fashioned maker in Metro Detroit, but moving onto bigger and better things in an industry with expected growth over 25% by 2023.


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August 31, 2002
Shatner vs the dorks
Chicago Sun-Times | Submitted by: Soylent
The great actor William Shatner, on his coming paintball career:
"I suggested to the guys running the game that I wanted to lead like a
leader, right up front. And then they did the mathematics for me.   Multiply 20 or 30 paintballs a second
times 1,400, and that's how many are coming my way. You know, leading
at front may not be the best idea."
|
|
Read article...
|
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Israel using flechettes
BBC | Submitted by: Zar
"The Israeli army confirmed it used flechettes in an operation near the
Jewish settlement of Netzarim, south of Gaza City, on Thursday. Palestinian
medical officials said the victims were cut to shreds after being hit by
the tiny metal darts, which are dispersed by a tank shell."
|
|
Read article...
|
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What First Amendment?
Sacramento Bee | Submitted by: Ann Onymous
For those who cherish the idea that America is free, this should come as a
shock: "The sentiment that the First Amendment goes too far was already on
the rise before the terrorist attacks a year ago, doubling to four in 10
between 2000 and 2001. The poll found that 49 percent think the First
Amendment goes too far, a total about 10 points higher than in 2001. 'Many
Americans view these fundamental freedoms as possible obstacles in the war
on terrorism,' said Ken Paulson, executive director of the First Amendment
Center, based in Arlington, Va., which commissioned the survey. Almost half
also said the media has been too aggressive in asking the government questions
about the war on terrorism."
|
|
Read article...
|
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The best playground for young boys ever
Global News | Submitted by: Gorg
"A fire that scorched a southwest Calgary playground Thursday has
neighbors concerned about the safety of the material covering the ground
there. The Glenbrook area playground is lined with recycled tires.
Investigators say a seven-year-old boy started the blaze by setting a
mattress on fire." WTF was a mattress doing in a children's
playground?"
|
|
Read article...
|
|
Taftquack
Dayton Daily News | Submitted by: Idle Hands
With only $375,000 in campaign funds at his disposal, the Democratic
gubernatorial candidate in Ohio, Tim Hagan, has launched an entirely
web-based offensive against current Ohio Republican governor Bob Taft.
The result: Taftquack, Bob taft's head placed on the body of a duck
and given a yellow bill, mimicking those annoying Aflac commercials.
Article includes web address to the commercial, which is just surreal
and absurd enough to win a few "the world is going to hell anyway so
why not help it along" votes.
|
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Read article...
|
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Thirteen graves washed away
Yahoo! Schlagzeilen | Submitted by: Alamar
After heavy raining, an graveyard of the City Siegen (in central Germany)
was washed away with 13 graves. Photo of some of the coffins headed
downstream. Also accompanied with an article in German.
|
|
Read article... and
This article...
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PMS can be a real bitch
Boston Herald | Submitted by: Mike C
"Two Malden women wielding a baseball bat and in the mood for 'causing
trouble' allegedly drove through four towns early yesterday morning,
bashing in the windows of some 40 cars before being caught by a cop
they nearly ran over... they are also suspected of having left a trail of
shattered glass in their wake across Reading, Wakefield and Stoneham."
|
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Read article...
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Hospital turns away patient because of fatness
WKMG-TV 6 Orlando | Submitted by: Rio
"A 326-pound man scheduled for a heart procedure was turned away from
an Ormond Beach, Fla., hospital because officials said he was too heavy
for the table. Instead of getting the outpatient surgery he needed,
Preston Bergin was sent home because the table in the lab wouldn't
hold him."
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Read article...
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Born criminal
Independent Online | Submitted by: Soylent
"According to court authorities in Dziezoniow, south-west Poland, Beniamin
drove away from a local wholesaler with a lorry-load of beer without paying a
cent in October last year. But Beniamin was born in November, as the infant's
grandmother pointed out to SuperExpress."
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Read article...
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Michael Jackson wins Britney Booby Prize
Sky News | Submitted by: Ryano
"[Britney Spears] said that, for her, Jackson was the artist of the
millennium. But Jackson took her words to mean he was being honoured as
the greatest singer of the last 1,000 years. The star then told the
audience and millions of TV viewers: "When I was a little boy growing
up in Indiana if someone told me I'd be getting the Artist of the
Millennium award, I'd never have believed it."
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Read article...
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| 651 | (A) | For-to affraye the folke that the defence made |
| 654 | (C) | To affraye þe folke . þat hem defens made |
| 646 | (D) | To affray þe folk þat defence made |
| 628 | (E) | to affraye þe folk þat defens made . |
| (Ex) | ---- no text ---- | |
| 669 | (L) | To affray þe folke þat defence made |
| 608 | (P) | To affray þe folke þat defence made |
| 637 | (U) | To affraien the folke / that defese made |
| (V) | ---- no text ---- |


Physician ℞Plorer
Visualizing the relationship between pharmaceutical company payments and drug prescriptions


ROBIN HOOD, PRINCE OF THIEVES [Spoilers] A film review by Laurie Mann Copyright 1991 Laurie Mann
The long-awaited, long-hyped version of Robin Hood hit the screens last night. Was it worth it?
Well, almost.
This version isn't as much fun as, say, the Errol Flynn version. It's a more serious, subdued, uneven movie. There is some wonderful photography, the sets look very medieval, and most of the costumes are pretty good. The main problems in the movie are the fault of the novice director (the only other movie this director had made was Heathers) and that script. Costner's accent problems pale beside these major flaws. Still, the movie is definitely worth viewing on the big screen. Once, anyway.
This movie opens in a hideous Islamic prison in Jerusalem. A reasonably illogical thing happens, permitting Robin Hood (Kevin Costner) and Azheem (Morgan Freeman) to escape. Back in England, Robin's father (an all-to-brief performance by Brian Blessed) is murdered by the sheriff of Nottingham (a scene-chewing performance by Alan Rickman) and his "merry" men.
Some of the best scenes in the entire movie are some sweeping shots of the English countryside as Robin watches Sir Guy of Gisborn set dogs on a 12-year-old boy. Robin, naturally, intercedes, killing a few knights and rescuing the boy.
Part of the problem with this movie is the far-too-campy performance by Alan Rickman as the sheriff, and the really awful lines he is given to say. Rickman, who played the memorable Hans in DIE HARD, was completely out-of-control in this movie. Costner gives a very earnest performance as Robin, and his accent is unbelievably inconsistent. Both Morgan Freeman and Mary Elizabeth Mastrantonio give the only really strong performances of the film, although the actors who play the thieves and their families were all fine.
Getting back to the script for a minute, anachronisms abound in the attitudes of the characters. While there were a few Moors in England at this time, given that the Crusades were in full force, it's unlikely Azheem would have wanted to go to England, or that he would have survived for very long once he was there. You have the sheriff talking about his "sanity," which was practically an unknown concept back at that time. You have a very odd speech about "nobility" that Robin gives, and a rousing, but equally odd speech given by Azheem about freedom. I don't expect screenwriters to have their characters speak in genuinely medieval English---it would have been almost unintelligible to the average film viewer. But I do expect screenwriters to have done enough research about the period and not give their characters unreasonable attitudes given the time.
The screenwriters did successfully portray the fact that the Moors were technologically superior to the English at that time. Azheem makes a few remarks about "savages," cooly assists at a difficult birth, and tries to teach Robin how to use a telescope. (Robin doesn't get it)
One other problem with this movie---though it's rated PG-13, it has a few really gross little twists in it. It's not a good movie for sensitive children (or adults!). There are also two attempted rapes, a hand amputation, hangings, various cuttings, and lots of sword & arrow play. The film editing was a little sloppy; about ten minutes could have been cut with no loss whatsoever.
Spoilers (but if you know the Robin Hood story, not really)
There are only two small surprises in this movie, but both were unbelievably telegraphed. There is something of a surprise at the very end of the movie. At least, it was a surprise to most of the audience, who started applauding and cheering for the first time in the entire film.
 This was a tired hack a few years ago. Why do screen writers persist on doing this???? Why do producers and directors let them?
Laurie Mann<EMAIL_ADDRESS>NeXT Mail<EMAIL_ADDRESS>.
The review above was posted to the
rec.arts.movies.reviews newsgroup (de.rec.film.kritiken for German reviews).
The Internet Movie Database accepts no responsibility for the contents of the
review and has no editorial control. Unless stated otherwise, the copyright
belongs to the author.
Please direct comments/criticisms of the review to relevant newsgroups.
Broken URLs inthe reviews are the responsibility of the author.
The formatting of the review is likely to differ from the original due
to ASCII to HTML conversion.
Related links: index of all rec.arts.movies.reviews reviews


Interact with trash directly by mouse enter and click. You can also force state:
intro
,
fill
,
erase
.


- GAIN - Static variable in class com.crickettechnology.audio.BiquadFilterParam
-
Gain in dB (for PEAK, LOW_SHELF, and HIGH_SHELF filter types)
- get() - Method in class com.crickettechnology.audio.FloatRef
-
Get the value
- get() - Method in class com.crickettechnology.audio.IntRef
-
Get the value
- get() - Method in class com.crickettechnology.audio.ObjectRef
-
Get the object
- get3dAttenuation(ObjectRef<AttenuationMode>, FloatRef, FloatRef, FloatRef) - Static method in class com.crickettechnology.audio.Sound
-
Gets parameters used for 3D volume attenuation.
- get3dListenerPosition(FloatRef, FloatRef, FloatRef, FloatRef, FloatRef, FloatRef, FloatRef, FloatRef, FloatRef) - Static method in class com.crickettechnology.audio.Sound
-
Gets the listener position and rotation in 3D space.
- get3dListenerVelocity(FloatRef, FloatRef, FloatRef) - Static method in class com.crickettechnology.audio.Sound
-
Gets the listener velocity in 3D space.
- get3dPosition(FloatRef, FloatRef, FloatRef) - Method in class com.crickettechnology.audio.Sound
-
Gets the position of the sound emitter in 3D space.
- get3dSoundSpeed() - Static method in class com.crickettechnology.audio.Sound
-
Gets the speed of sound.
- get3dVelocity(FloatRef, FloatRef, FloatRef) - Method in class com.crickettechnology.audio.Sound
-
Gets the velocity of the sound emitter in 3D space.
- getChannels() - Method in class com.crickettechnology.audio.Sound
-
Gets the number of channels in the sound (1 for mono, 2 for stereo).
- getClipFlag() - Static method in class com.crickettechnology.audio.Ck
-
Returns the value of the clip flag.
- getCurrentLoop() - Method in class com.crickettechnology.audio.Sound
-
Gets the number of the current loop.
- getGlobalEffectBus() - Static method in class com.crickettechnology.audio.EffectBus
-
Get the global effect bus.
- getLength() - Method in class com.crickettechnology.audio.Sound
-
Gets the total duration of the sound, in sample frames.
- getLengthMs() - Method in class com.crickettechnology.audio.Sound
-
Gets the total duration of the sound, in milliseconds.
- getLoop(IntRef, IntRef) - Method in class com.crickettechnology.audio.Sound
-
Get the loop start and end.
- getLoopCount() - Method in class com.crickettechnology.audio.Sound
-
Gets the number of times the sound should loop.
- getMaster() - Static method in class com.crickettechnology.audio.Mixer
-
Returns the master mixer, which is the root of the mixer hierarchy.
- getMixedPauseState() - Method in class com.crickettechnology.audio.Mixer
-
Returns true if this mixer, or any of its ancestors, is paused.
- getMixedPauseState() - Method in class com.crickettechnology.audio.Sound
-
Returns true if the sound is pauses, its mixer is paused, or any of its
mixer's ancestors are paused.
- getMixedVolume() - Method in class com.crickettechnology.audio.Mixer
-
Returns the volume used for mixing (affected by ancestors).
- getMixedVolume() - Method in class com.crickettechnology.audio.Sound
-
Gets the volume value used for mixing.
- getMixer() - Method in class com.crickettechnology.audio.Sound
-
Gets the mixer to which this sound is assigned.
- getName() - Method in class com.crickettechnology.audio.Bank
-
Returns the name of the bank.
- getName() - Method in class com.crickettechnology.audio.Mixer
-
Returns the name of the mixer.
- getNextSound() - Method in class com.crickettechnology.audio.Sound
-
Gets the sound to be played when this sound finishes playing.
- getNumSounds() - Method in class com.crickettechnology.audio.Bank
-
Returns the number of sounds in the bank.
- getOutputBus() - Method in class com.crickettechnology.audio.EffectBus
-
Get the bus to which this bus's output is sent.
- getPan() - Method in class com.crickettechnology.audio.Sound
-
Gets the pan.
- getPanMatrix(FloatRef, FloatRef, FloatRef, FloatRef) - Method in class com.crickettechnology.audio.Sound
-
Gets the pan matrix.
- getParent() - Method in class com.crickettechnology.audio.Mixer
-
Returns the parent of the mixer.
- getPitchShift() - Method in class com.crickettechnology.audio.Sound
-
Gets the pitch shift value, in half-steps.
- getPlayPosition() - Method in class com.crickettechnology.audio.Sound
-
Get the current play position in the sound, in sample frames.
- getPlayPositionMs() - Method in class com.crickettechnology.audio.Sound
-
Get the current play position in the sound, in milliseconds.
- getRenderLoad() - Static method in class com.crickettechnology.audio.Ck
-
Returns an estimate of the load on the audio processing thread.
- getSampleRate() - Method in class com.crickettechnology.audio.Sound
-
Gets the sample rate of the sound, in Hz.
- getSoundName(int) - Method in class com.crickettechnology.audio.Bank
-
Returns the name of a sound in this bank.
- getSpeed() - Method in class com.crickettechnology.audio.Sound
-
Gets the playback speed.
- getVolume() - Method in class com.crickettechnology.audio.Mixer
-
Get the volume of this mixer.
- getVolume() - Method in class com.crickettechnology.audio.Sound
-
Gets the volume.
- getVolumeRampTime() - Static method in class com.crickettechnology.audio.Ck
-
Gets the maximum rate at which volumes change on playing sounds, in ms per full volume scale.
- getWetDryRatio() - Method in class com.crickettechnology.audio.Effect
-
Gets the wet/dry ratio for this effect.
- getWetDryRatio() - Method in class com.crickettechnology.audio.EffectBus
-
Gets the wet/dry ratio for this bus.
- init(Context) - Static method in class com.crickettechnology.audio.Ck
-
Initializes Cricket Audio.
- init(Context, Config) - Static method in class com.crickettechnology.audio.Ck
-
Initializes Cricket Audio.
- IntRef - Class in com.crickettechnology.audio
-
Mutable integer container, used for emulating int C++ reference parameters.
- IntRef() - Constructor for class com.crickettechnology.audio.IntRef
-
- InvDistance - Static variable in class com.crickettechnology.audio.AttenuationMode
-
Attenuation proportional to the reciprocal of the distance.
- InvDistanceSquared - Static variable in class com.crickettechnology.audio.AttenuationMode
-
Attenuation proportional to the square of the reciprocal of the distance.
- is3dEnabled() - Method in class com.crickettechnology.audio.Sound
-
Gets whether 3D positioning is enabled.
- isBypassed() - Method in class com.crickettechnology.audio.Effect
-
Gets whether this effect is bypassed.
- isBypassed() - Method in class com.crickettechnology.audio.EffectBus
-
Gets whether this bus is bypassed.
- isFailed() - Method in class com.crickettechnology.audio.Bank
-
Returns true if an asynchronously loaded bank had an error during loading.
- isFailed() - Method in class com.crickettechnology.audio.Sound
-
Returns true if an error occurred while creating the sound.
- isLoaded() - Method in class com.crickettechnology.audio.Bank
-
Returns true when an asynchronously loaded bank has finished loading.
- isLoopReleased() - Method in class com.crickettechnology.audio.Sound
-
Returns true if releaseLoop() has been called after the last call to play().
- isPaused() - Method in class com.crickettechnology.audio.Mixer
-
Get whether this mixer is paused.
- isPaused() - Method in class com.crickettechnology.audio.Sound
-
Gets whether the sound is paused.
- isPlaying() - Method in class com.crickettechnology.audio.Sound
-
Gets whether the sound is playing.
- isReady() - Method in class com.crickettechnology.audio.Sound
-
Returns true if the sound is ready.
- isVirtual() - Method in class com.crickettechnology.audio.Sound
-
Gets whether a 3D sound is virtual.
- newBank(String) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank from an asset in the .apk.
- newBank(String, PathType) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank.
- newBank(String, PathType, int, int) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank.
- newBankAsync(String) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank asynchronously from an asset in the .apk.
- newBankAsync(String, PathType) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank asynchronously.
- newBankAsync(String, PathType, int, int) - Static method in class com.crickettechnology.audio.Bank
-
Loads a bank asynchronously.
- newBankSound(Bank, int) - Static method in class com.crickettechnology.audio.Sound
-
Creates a sound from a bank by index.
- newBankSound(Bank, String) - Static method in class com.crickettechnology.audio.Sound
-
Creates a sound from a bank by name.
- newCustomEffect(int) - Static method in class com.crickettechnology.audio.Effect
-
Create a custom effect (previously registered with the C++ API).
- newEffect(EffectType) - Static method in class com.crickettechnology.audio.Effect
-
Create a built-in effect.
- newEffectBus() - Static method in class com.crickettechnology.audio.EffectBus
-
Create a new effect bus.
- newMixer(String, Mixer) - Static method in class com.crickettechnology.audio.Mixer
-
Creates a new mixer.
- newMixer(String) - Static method in class com.crickettechnology.audio.Mixer
-
Creates a new mixer.
- newStreamSound(String) - Static method in class com.crickettechnology.audio.Sound
-
Creates a streaming sound from an asset in the .apk.
- newStreamSound(String, PathType) - Static method in class com.crickettechnology.audio.Sound
-
Creates a streaming sound.
- newStreamSound(String, PathType, int, int, String) - Static method in class com.crickettechnology.audio.Sound
-
Creates a streaming sound.
- None - Static variable in class com.crickettechnology.audio.AttenuationMode
-
No attenuation; volume is 1.0 when closer than the far distance, and far volume outside of that.
- NOTCH - Static variable in class com.crickettechnology.audio.BiquadFilterParam_FilterType
-
Notch filter blocks a narrow band of frequencies.
- set(float) - Method in class com.crickettechnology.audio.FloatRef
-
Set the value
- set(int) - Method in class com.crickettechnology.audio.IntRef
-
Set the value
- set(T) - Method in class com.crickettechnology.audio.ObjectRef
-
Set the object
- set3dAttenuation(AttenuationMode, float, float, float) - Static method in class com.crickettechnology.audio.Sound
-
Sets parameters used for 3D volume attenuation.
- set3dEnabled(boolean) - Method in class com.crickettechnology.audio.Sound
-
Sets whether 3D positioning is enabled.
- set3dListenerPosition(float, float, float, float, float, float, float, float, float) - Static method in class com.crickettechnology.audio.Sound
-
Sets the listener position and rotation in 3D space.
- set3dListenerVelocity(float, float, float) - Static method in class com.crickettechnology.audio.Sound
-
Sets the listener velocity in 3D space.
- set3dPosition(float, float, float) - Method in class com.crickettechnology.audio.Sound
-
Sets the position of the sound emitter in 3D space.
- set3dSoundSpeed(float) - Static method in class com.crickettechnology.audio.Sound
-
Sets the speed of sound.
- set3dVelocity(float, float, float) - Method in class com.crickettechnology.audio.Sound
-
Sets the velocity of the sound emitter in 3D space.
- setBypassed(boolean) - Method in class com.crickettechnology.audio.Effect
-
Set whether this effect is bypassed.
- setBypassed(boolean) - Method in class com.crickettechnology.audio.EffectBus
-
Set whether this bus is bypassed.
- setEffectBus(EffectBus) - Method in class com.crickettechnology.audio.Sound
-
Sets the effect bus to which this sound's audio is sent to be processed, or
null for dry output (no effects).
- setLoop(int, int) - Method in class com.crickettechnology.audio.Sound
-
Set the loop start and end.
- setLoopCount(int) - Method in class com.crickettechnology.audio.Sound
-
Sets the number of times the sound should loop.
- setMixer(Mixer) - Method in class com.crickettechnology.audio.Sound
-
Sets the mixer to which this sound is assigned.
- setName(String) - Method in class com.crickettechnology.audio.Mixer
-
Sets the name of the mixer.
- setNextSound(Sound) - Method in class com.crickettechnology.audio.Sound
-
Sets the sound to be played when this sound finishes playing.
- setOutputBus(EffectBus) - Method in class com.crickettechnology.audio.EffectBus
-
Set the bus to which this bus's output is sent.
- setPan(float) - Method in class com.crickettechnology.audio.Sound
-
Sets the pan.
- setPanMatrix(float, float, float, float) - Method in class com.crickettechnology.audio.Sound
-
Sets the pan matrix explicitly.
- setParam(int, float) - Method in class com.crickettechnology.audio.Effect
-
Set an effect parameter.
- setParent(Mixer) - Method in class com.crickettechnology.audio.Mixer
-
Sets the parent of the mixer.
- setPaused(boolean) - Method in class com.crickettechnology.audio.Mixer
-
Set whether this mixer is paused.
- setPaused(boolean) - Method in class com.crickettechnology.audio.Sound
-
Sets whether the sound is paused.
- setPitchShift(float) - Method in class com.crickettechnology.audio.Sound
-
Sets the pitch shift value, in half-steps.
- setPlayPosition(int) - Method in class com.crickettechnology.audio.Sound
-
Set the play position in the sound, in sample frames.
- setPlayPositionMs(float) - Method in class com.crickettechnology.audio.Sound
-
Set the play position in the sound, in milliseconds.
- setSpeed(float) - Method in class com.crickettechnology.audio.Sound
-
Sets the playback speed.
- setVolume(float) - Method in class com.crickettechnology.audio.Mixer
-
Set the volume of this mixer.
- setVolume(float) - Method in class com.crickettechnology.audio.Sound
-
Sets the volume.
- setVolumeRampTime(float) - Static method in class com.crickettechnology.audio.Ck
-
Sets the maximum rate at which volumes change on playing sounds, in ms per full volume scale.
- setWetDryRatio(float) - Method in class com.crickettechnology.audio.Effect
-
Sets the wet/dry ratio for this effect.
- setWetDryRatio(float) - Method in class com.crickettechnology.audio.EffectBus
-
Sets the wet/dry ratio for this bus.
- shutdown() - Static method in class com.crickettechnology.audio.Ck
-
Shuts down Cricket Audio.
- Sound - Class in com.crickettechnology.audio
-
A sound (either memory-resident or streamed).
- SOUND_SPEED_CENTIMETERS_PER_SECOND - Static variable in class com.crickettechnology.audio.Sound
-
The speed of sound in dry air at 20 degrees C, in centimeters per second.
- SOUND_SPEED_FEET_PER_SECOND - Static variable in class com.crickettechnology.audio.Sound
-
The speed of sound in dry air at 20 degrees C, in feet per second.
- SOUND_SPEED_INCHES_PER_SECOND - Static variable in class com.crickettechnology.audio.Sound
-
The speed of sound in dry air at 20 degrees C, in inches per second.
- SOUND_SPEED_METERS_PER_SECOND - Static variable in class com.crickettechnology.audio.Sound
-
The speed of sound in dry air at 20 degrees C, in meters per second.
- startCapture(String, PathType) - Static method in class com.crickettechnology.audio.Ck
-
Start capturing the final audio output to a file.
- stop() - Method in class com.crickettechnology.audio.Sound
-
Stop the sound.
- stopCapture() - Static method in class com.crickettechnology.audio.Ck
-
Stop capturing audio output.
- StreamBufferMs - Variable in class com.crickettechnology.audio.Config
-
Size of buffer for stream sounds.
- StreamFileUpdateMs - Variable in class com.crickettechnology.audio.Config
-
Interval between file reads for stream sounds, in ms.
- suspend() - Static method in class com.crickettechnology.audio.Ck
-
Suspends Cricket Audio.


This FreeDOS browser example is powered by v86. This specific sample was created for the FreeDOS Boot Disks project.


We consist of three people. Two engineers and the third is our business manager
ROSYAM is a company specialized in engineering projects related to the field of software, including the field of developing homes into more secure and sophisticated smart homes.
Lorem ipsum dolor amet, consecte adipiscing elit, quisque dictum convallis mi. In dapibus auctor dictum donec mattis quis eros ultricies feugiat. Morbi congue faucibus mi, ague blandit curabitur ac lacinia.
- artificial intelligence
- Web Design
- designing a logo
- Android Apps
- Electronics
- Cyber Security


Dear student,
You have been invited to join {{title}} at {{institution}} by a member of the
course staff.
To finish your registration, please visit {{registration_url}} and fill out the
registration form.
Once you've registered, to start accessing course materials, please visit
{{course_url}}.
Thanks!
{{institution}}
----
This email was automatically sent from {{institution}} to {{email}}.


Av. Eugenio Garza Sada 2501 Sur
Monterrey, 64849
Nuevo Leon,
Mexico
Sed do eiusmod tempor incididunt ut labore et dolore magna aliqua.
+52 8110 8000
Please feel free to write an email to us or to use our electronic ticketing system.
Travel Studio does not maintain a list of people who send messages. The details of the correspondents are kept only for the time needed to process their messages. Travel Studio does not sell or pass on such data to any third party .


The audio stream is:
o Recorded using live-audio
input.
o Filtered using an HP filter with fc=1500 Hz.
o Encoded using
Opus.
o Transmitted (in loopback) to remote peer using
RTCPeerConnection where it is decoded.
o Finally, the received remote stream is used as source to an <audio>
tag and played out locally.
Press any key to add an effect to the transmitted audio while talking.
Please note that:
o Linux is currently not supported.
o Sample rate and channel configuration must be the same for input and
output sides on Windows.
o Only the Default microphone device can be used for capturing.
For more information, see WebRTC integration with the Web Audio API.


Discover exciting third level education opportunities at Letterkenny Institute of Technology.
We have two thriving campuses in Ireland's North West: Letterkenny and Killybegs.
Our Schools of Business, Engineering, Science and Tourism provide a wide variety of programmes, with awards right up to Masters and Doctorate level.
| Blah |
$("[id]").css("border", "1px solid red") |
$("table[border=1]").css("border", "1px solid red") |


p
p class "first"
In this example, we return all elements with class names "first", "second" and "third", that are the next sibling elements between h2 and h5.


1) So a successfull store isn't enough of a reason to stop sending data because of a) DHT churn (network loosing nodes, getting new ones), b) shifts in the underlying network and changes in DHT routing, c) possibility of interference (when someone's writing to the same spot and we want to compete for it until the recipient gets the data), d) possibility of the recipient restarting or changing states, so even if it gets the data, it might need to get it again.
2) 3) One possibility is that ...


{% extends 'portal.html' %} {% load static %} {% block breadcrumbs %} {% endblock breadcrumbs %} {% block main_content %}
Special Unicode characters such as ♂ or ♀ are allowed to be used throughout HAWC, including data visualizations. However, when importing data from existing spreadsheets, the input file must be saved in a compatible format to display these characters. When using Excel, a spreadsheet with data for export could be structured in the following way:
Excel File Example, with Unicode characters for sex specification.
To export for HAWC, in the Save-As dialog, select "Unicode Text":
Excel 2010 Save As Dialog Box. Select the "Unicode Text" option for exporting data for use in HAWC.
The data can now be imported for use in HAWC.


Add {% load uni_form_tags %} to the template that calls your form.
Append your form call with the as_uni_form filter:
{{ my_form|as_uni_form }}
Add the class of ‘uniForm’ to your form. Example:
<form action="" method="post" class="uniForm">
Refresh and enjoy!
In your views.py add the following after field definitions:
from django.shortcuts import render_to_response
from uni_form.helpers import FormHelper, Submit, Reset
from my_project.forms.MyForm
def my_view(request):
# Create the form
form = MyForm()
# create a formHelper
helper = FormHelper()
# Add in a class and id
helper.form_id = 'this-form-rocks'
helper.form_class = 'search'
# add in a submit and reset button
submit = Submit('search','search this site')
helper.add_input(submit)
reset = Reset('reset','reset button')
helper.add_input(reset)
# create the response dictionary
response_dictionary = {'form':form, 'helper': helper}
return render_to_response('my_template.html', response_dictionary)
In your template do the following:
{% load uni_form_tags %}
{% uni_form form helper %}
In your form class add the following after field definitions:
from uni_form.helpers import FormHelper, Submit, Reset
class MyForm(forms.Form):
title = forms.CharField(label=_("Title"), max_length=30, widget=forms.TextInput())
# Attach a formHelper to your forms class.
helper = FormHelper()
# Add in a class and id
helper.form_id = 'this-form-rocks'
helper.form_class = 'search'
# add in a submit and reset button
submit = Submit('search','search this site')
helper.add_input(submit)
reset = Reset('reset','reset button')
helper.add_input(reset)
In your template do the following:
{% load uni_form_tags %}
{% with form.helper as helper %}
{% uni_form form helper %}
{% endwith %}
In your form class add the following after field definitions:
from uni_form.helpers import FormHelper, Submit
class MyForm(forms.Form):
title = forms.CharField(label=_("Title"), max_length=30, widget=forms.TextInput())
# Attach a formHelper to your forms class.
helper = FormHelper()
# Change the form and method
helper.form_action = 'my-url-name-defined-in-url-conf'
helper.form_method = 'GET' # Only GET and POST are legal
# add in a submit and reset button
submit = Submit('search','search this site')
helper.add_input(submit)
In your template do the following:
{% load uni_form_tags %}
{% with form.helper as helper %}
{% uni_form form helper %}
{% endwith %}
Uniform helpers can use layout objects. A layout can consist of fieldsets, rows, columns, HTML and fields. A simple Example:
from django import forms
from uni_form.helpers import FormHelper, Submit, Reset
from uni_form.helpers import Layout, Fieldset, Row, HTML
class LayoutTestForm(forms.Form):
is_company = forms.CharField(label="company", required=False, widget=forms.CheckboxInput())
email = forms.CharField(label="email", max_length=30, required=True, widget=forms.TextInput())
password1 = forms.CharField(label="password", max_length=30, required=True, widget=forms.PasswordInput())
password2 = forms.CharField(label="re-enter password", max_length=30, required=True, widget=forms.PasswordInput())
first_name = forms.CharField(label="first name", max_length=30, required=True, widget=forms.TextInput())
last_name = forms.CharField(label="last name", max_length=30, required=True, widget=forms.TextInput())
# Attach a formHelper to your forms class.
helper = FormHelper()
# Create some HTML that you want in the page.
# Yes, in real life your CSS would be cached, but this is just a simple example.
style = """
<style>
.formRow {
color: red;
}
</style>
"""
# create the layout object
layout = Layout(
# first fieldset shows the company
Fieldset('', 'is_company'),
# second fieldset shows the contact info
Fieldset('Contact details',
HTML(style),
'email',
Row('password1','password2'),
'first_name',
'last_name',
)
)
helper.add_layout(layout)
submit = Submit('add','Add this contact')
helper.add_input(submit)
Then, just like in the previous example, add the following to your template:
{% load uni_form_tags %}
{% with form.helper as helper %}
{% uni_form form helper %}
{% endwith %}
This allows you to group fields in fieldsets, or rows or columns or add HTML between fields, etc.


This section documents the base services of the windowing subsystem in BW. The base services provide a level of functionality approximately equivalent to Xlib in X Windows. That is: manipulation of basic windows, drawing primitives, fonts, colours, bitmaps, events, keyboard focus, and clipping regions.
This document Is not a specification, but merely indicates a direction and is used as a cross check on the actual design. Only the portions of this functionality actually used by some BW application (or a reasonable extension of one) will be implemented. This is consistent with the concept that BW is an isolation layer for a set of applications and not a general isolation layer for any arbitrary graphic application.
This design work provides the framework so that new features can be added consistently over time as they are required for BW applications. It is also a cross check on the design because it's completeness can be used to identify design alternatives that are insufficiently general to be expandable in ways known to be needed by some applications.
Because it cross references the two primary platforms for delivery of BW, it highlights routines that may have to be added to one of the platforms in the implementation.
These calls are based on a analysis of the calls and events in WIN32 and Xlib. These lists are "complete" in the following sense: All of the Xlib calls are listed and for each Xlib call a search was made for WIN32 call(s) that provide the a similar service.
See the detailed documentation (extracted from the source) for specifics on which routines are available, their parameters, and notes on their usage.
The objects covered by this portion of the specification are:
Not included here are:


-
$color-neutral-white
#fff
-
$color-neutral-gray-02
#f9f9f9
-
$color-neutral-gray-07
#eee
-
$color-neutral-gray-13
#ddd
-
$color-neutral-gray-27
#bbb
-
$color-neutral-gray-50
#808080
-
$color-neutral-gray-60
#666
-
$color-neutral-gray-73
#444
-
$color-neutral-gray-93
#131313
-
$color-neutral-black
#000
To add to these items, use Sass variables that start with $color-neutral-
in ./source/css/scss/abstracts/_variables.scss


§183‑18 Criminal penalties. Any person who violates section 183-17, upon conviction thereof, is guilty of a misdemeanor and shall be fined not more than $2,000 or imprisoned not more than one year, or both. In addition to any other penalty imposed under this section, a fine of up to $2,000 shall be levied for each tree illegally destroyed or harvested under section 183‑17. [L 1919, c 83, §2; RL 1925, §596; RL 1935, §185; RL 1945, §1020; RL 1955, §19-9; HRS §183-18; am L 1981, c 85, §6; am L 2006, c 174, §2]
Cross References
General administrative penalties, see §183-5.


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On the first full day this page went live, 100,000 people visited this Rotten
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May 05, 2003
White House concedes no WMD in Iraq
Glasgow Sunday Herald | Submitted by: JRS
"The Bush administration has admitted that Saddam Hussein probably had no weapons of mass destruction. Senior officials in the Bush administration have admitted that they would be 'amazed' if weapons of mass destruction (WMD) were found in Iraq.  Somebody's got some 'splaining to do.
|
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Read article...
|
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Human shields beginning to smell the coffee
Wire Services | Submitted by: Bstar
"Young Western activists living with Palestinians to act as 'human shields' against Israeli raids are debating how to minimize their risk of dying after suffering a sudden rash of casualties." Evidently they must have believed that their mere presence would be enough to deter Israel's military operations. Not very bright.
|
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Read article...
|
|
Deathcow unleashed by psilocybin
palmbeachpost.com | Submitted by: optional
"A motorcyclist died early Monday when he hit one of about a dozen cows that wandered onto Interstate 95 near the Hobe Sound interchange... The cow was also killed in the crash. Investigators will try to determine who cut the fence. There has been a problem in Martin County and other rural areas in recent years with people cutting fences on cattle grazing land to get access to psychedelic mushrooms that grow around the cow manure."
|
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Read article...
|
|
Indians getting high off scorpion stings
News Corporation | Submitted by: Johnny Cage
"The trendy and the elite in India's wealthy western state of Gujarat, bored with mundane drugs, are turning to the sting of a scorpion to get their kicks... After the customer pays a fee of 150 to 200 rupees (about $A5-7) the scorpion is produced and placed on the body of the thrill-seeker, who is then viciously stung. Users say after the initial pain the venom produces an illusionary, floating feeling." In the words of one arachnid pusher: "You should try it at least once ... a lifetime experience."
|
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Read article...
|
|
The world's most primitive car bomb
ABC TV affiliates | Submitted by: Reid Fleming
"Authorities believe a man who died in a fiery explosion in the town square filled his car with open buckets of gasoline and drove toward the workplace of his wife, who was trying to divorce him... The car contained at least six buckets filled with gasoline."  "He started taunting him, saying, 'I'm not going to pay you, and there is nothing you can do about it.' He knew he wasn't going to call the cops on a $5 fare."  There's something poetic in that.
|
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Read article...
|
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Maine's cadaver glut
Lewiston Sun-Journal | Submitted by: Reid Fleming
"Eighty cadavers are waiting in refrigerated storage on campus at the University of New England in Biddeford. ... The school has had to get choosier so it doesn't run out of storage space. [They] stopped taking donors who are extremely obese -- bodies not considered ideal for anatomy studies. They already refused cadavers with HIV, tuberculosis and severe trauma at death."
|
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Read article...
|
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Iraqis tried to hand over Pvt. Jessica Lynch
Toronto Star | Submitted by: Sniffer
"'The Iraqi soldiers and commanders had left the hospital almost two days earlier,' Houssona said. 'The night they left, a few of the senior medical staff tried to give Jessica back. We carefully moved her out of intensive care and into an ambulance and began to drive to the Americans, who were just one kilometre away. But when the ambulance got within 300 metres, they began to shoot.'"
|
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Read article...
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Gang of transvestite robbers plaguing Manila
Independent Online | Submitted by: Tim Howard
"Philippines police on Monday warned taxi drivers in the capital against a gang of gay robbers who not only steal money but kisses from their victims as well. Police officer Anthony Cordova said taxi driver Rolando Estacio fell victim to three homosexual men who robbed him on Sunday at Manila's port area. Cordova said Estacio did not suspect his passengers, who hailed his taxi outside a shopping mall and asked to be brought to the port area, because 'they were all sweet and effeminate.'"
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Read article...
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Hiker's arm recovered, taken to funeral home
AZ Central | Submitted by: Tim Howard
"Thirteen park rangers lugged equipment into this southeastern Utah park and lifted an 800-pound boulder that had trapped a mountain climber until he amputated his arm with a pocketknife... The lower portion of Ralston's right arm was recovered Sunday and taken to a mortuary." Yes, but will there be an open casket at the viewing?
|
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Read article...
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Imbecile's rapist unidentifiable
Chicago Sun-Times | Submitted by: Tim Howard
"A mentally disabled woman in a group home is more than five months pregnant from a rape, but identification of her attacker has been hampered because she is unable to consent to a DNA test. The 22-year-old woman, who has no family, is too disabled to speak. She was abandoned as a child and has lived at the small home for 19 years. 'She cannot even say yes or no,' Detective Rick Salcedo said.  He dotes on his nieces and nephews. He admires Colin Powell and Abraham Lincoln. In his holster is a Walther PPK, James Bond's preferred pistol. And for the past six months, ever since he made a public announcement that angered some and inspired others in this small Bay Area city 50 miles northeast of San Francisco, Ron Forsythe has been the only openly gay police chief in the nation."
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Read article...
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Chicken farmers get grief counseling
Sacramento Bee | Submitted by: Tim Howard
"A state and federal task force has begun to offer grief therapy to poultry owners whose birds were killed in order to stave off the spread of Exotic Newcastle Disease. The task force is also providing stress counseling and sensitivity training to its field workers who deal with the deadly avian disease."
|
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Read article...
|
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Cosmonauts packing heat -- just in case
CNN | Submitted by: RedBeagle
"Eleven years before, two cosmonauts overshot their touchdown site by 2,000 miles and found themselves deep in a forest with hungry wolves. That's when Russian space officials decided to pack a sawed-off shotgun aboard every spacecraft."
|
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Read article...
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--- title: core functions for video keywords: fastai sidebar: home_sidebar summary: "API details." description: "API details."


Offers a gene-based meta-analysis test with filtering to detect gene-environment interactions (GxE) with association data, proposed by Wang et al. (2018) <doi:10.1002/gepi.22115>. It first conducts a meta-filtering test to filter out unpromising SNPs by combining all samples in the consortia data. It then runs a test of omnibus-filtering-based GxE meta-analysis (ofGEM) that combines the strengths of the fixed- and random-effects meta-analysis with meta-filtering. It can also analyze data from multiple ethnic groups.
| Version: | 1.0 |
| Depends: | CompQuadForm, MASS, forestplot |
| Published: | 2018-07-13 |
| Author: | Jiebiao Wang, Qianying Liu and Lin S. Chen |
| Maintainer: | Jiebiao Wang <randel.wang at gmail.com> |
| BugReports: | https://github.com/randel/ofGEM/issues |
| License: | GPL-2 | GPL-3 [expanded from: GPL] |
| URL: | https://github.com/randel/ofGEM |
| NeedsCompilation: | no |
| In views: | MetaAnalysis |
| CRAN checks: | ofGEM results |
| Reference manual: | ofGEM.pdf |
| Package source: | ofGEM_1.0.tar.gz |
| Windows binaries: | r-devel: ofGEM_1.0.zip, r-release: ofGEM_1.0.zip, r-oldrel: ofGEM_1.0.zip |
| OS X binaries: | r-release: ofGEM_1.0.tgz, r-oldrel: ofGEM_1.0.tgz |
Please use the canonical form https://CRAN.R-project.org/package=ofGEM to link to this page.


Memoree
- Minimal UX/UI design for iOS
SNS-like application, but the contents inside 'Memoree' are all private.
You can post an image with a line of quote(2 lines max on screen
for data-balloon-pos="down" data-balloon, 3 lines for paragraph)
on your wall, which no one can access on it.
The only way to show your posts to others is to share your post
via other SNS services.
Selected as an ADAA 2018 Semifinalist
Click here to watch this project on Behance
Click here to try clickable mockup (Adobe XD)
Title :
Memoree - Minimal UX/UI design for iOS
Date : August 27, 2017 (1 Week)
Tools : Adobe Experience Design CC 2017
Designer : Hyouk Seo (Spemer)


My name is sankalpa baral, I am 14years old web developer from Biratnagar,Nepal I love to do coding So Now a days I am focusing in my website.


You might think airports are boring – nothing more than an unavoidable waypoint on a journey to your final destination. Let us convince you otherwise by sending you on a TravelQuest Safari!
On a TravelQuest Safari, we let you hunt QR codes, which we hid throughout FRAPORT. The further away the treasures, the more points you get for finding them. And the more points you hunt, the better the rewards you get in our various airport shops!

The dialog box is different from a normal page, it is displayed on top of the current page and it will not span the entire width of the page. The dialog has also an icon of "X" in the header to close the box.
Go to Page One


Fungi are eukaryotic organisms present ubiquitously in our surroundings. Though many fungal species are harmless, some fungi can wreak havoc in immunocompromised patients, and are hence referred to as “opportunistic” pathogens. Opportunistic invasive fungal infections (IFIs), like mucormycosis, aspergillosis, and candidiasis, are becoming more prevalent with the onset of immunosuppressive therapies, organ transplantation, etc. In such settings, patients are treated with immunosuppressant drugs to prevent graft rejection by the host, which weakens the body’s natural defence mechanisms against fungal pathogens. Similarly, patients suffering from diseases such as AIDS are also at high risk due to their weakened immune systems. Recently, due to the COVID-19 pandemic, the number of cases of such infections have skyrocketed, especially in India.
Annually, over 150 million cases of fungal infections are reported globally, with upto 1.7 million deaths per year. IFIs also carry a 60-90% mortality rate among all ICU patients. Although cases of fungal infections have been on the rise, it wasn’t until the wake of Covid-19 that this issue was brought up in the media. Hence fungal infections are sometimes also quoted as a silent crisis.
Fungi, being eukaryotes, share a greater degree of molecular similarity to humans than prokaryotic pathogens such as bacteria. Thus, it is difficult to find unique molecular drug targets which will selectively affect the fungal pathogen and not the host. In the limited instances where such targets are available, there is always the looming threat of emergence of drug resistant fungal strains. Hence, developing strategies to tackle the emergence of drug resistant fungal strains is currently of paramount importance.
It is for this reason that we chose to target chitin (a long chain polymer of N-acetylglucosamine), an essential component of the cell walls of all fungal species, notably absent from eukaryotic cells such as our own. Our molecular weapons are ‘chitinases’, naturally occurring enzymes known to break down the glycosidic bonds in chitin. These are often present in bacterial and plant species, who use them as defence mechanisms against invading fungal pathogens (is this correct, source?). However, given the diversity in fungal species and chitinase enzymes, selecting the appropriate chitinase on a case to case basis would be quite difficult. Our aim is to harness the powers of these naturally occurring chitinase enzymes by generating strategic combinations of their functional domains, to combine their substrate specificities and possibly even activity. In this manner, we propose the development of a defined set of recombinant chitinase enzymes, combining the bets attributes from the wild type enzymes and thus be more efficient.
Importantly, the chitin biosynthesis pathway in fungi is rather complex and involves the concerted action of several enzymes in a well-regulated fashion. Hence, we hypothesise that the probability of alteration of chitin structure by the target organisms to avoid lysis by our chitinases is rather low. Our enzyme design combines functional domains from diverse chitinases, essentially combining the best attributes of each enzyme into one. The recombinant proteins are backed up by an extensive literature survey supporting the feasibility of the project.
Therefore, we are developing a novel, eco-friendly and broad-spectrum therapeutic ‘MOLDEMORT’ to tackle various invasive fungal infections. This solution has potential wide-ranging applications in healthcare, agriculture, and society alike.
Figure 1. This is a placeholder image. I hope igem folks got better stuff.
The project idea surfaced when a group of keen-eyed students observed fungal contamination in various locations, both inside and outside our campus. Background reading on pathogenic fungal species revealed to us that that Fusarium sp. (causes eye infections), Rhizopus sp. (causes mucormycosis in COVID-19 patients), Aspergillus sp. (causes aspergillosis in COVID-19 patients), etc. are widely present in many public places and often cause complications for immunocompromised patients. Armed with this information on the pathogenic nature of certain fungal species, we decided to generate pure cultures and try to identify the species present in our campus, and were able to identify species such as (add here).
We then grew interested in the question of how fungal infections caused by these species and others could be treated. A survey of literature revealed that the current antifungals used for treatment include various compounds such as amphotericin B (AmB), caspofungin, triazoles etc., but these antifungal agents have severe limitations due to lack of sufficient fungicidal effect and toxic side effects. Along with this, there have also been several reports of the emergence of drug resistant fungal strains due to the limited molecular targets of the existing drugs.
It was here that we decided to step in and try to design a novel antifungal agent targeting a nearly ubiquitous component of fungal cell walls, the polymer chitin. Our hypothesis is that lysis of chitin present in the cell walls of fungi by the naturally occurring chitinase enzymes will compromise the integrity of fungal cells, leading to their death. By harnessing the power of synthetic biology and protein engineering, the team IISER_TVM has engineered a chimeric chitinase to tackle the issue of invasive fungal infections.