Class PictureparkRetryHandler
Decorating
Inheritance
Namespace: System.Dynamic.ExpandoObject
Syntax
public class PictureparkRetryHandler : DelegatingHandler
Constructors
PictureparkRetryHandler(HttpMessageHandler, Int32)
Initializes a new instance of the PictureparkRetryHandler class to handle throttled requests (HTTP 429 Too many requests). Adds an inner handler for further processing.
Declaration
public PictureparkRetryHandler(HttpMessageHandler innerHandler, int maxRetries = 3)
Parameters
|
HttpMessageHandler
innerHandler
The inner handler. |
|
System.Int32
maxRetries
Maximum number of retries before a request is failing. |
PictureparkRetryHandler(Int32)
Initializes a new instance of the PictureparkRetryHandler class to handle throttled requests (HTTP 429 Too many requests).
Uses the default
Declaration
public PictureparkRetryHandler(int maxRetries = 3)
Parameters
|
System.Int32
maxRetries
Maximum number of retries before a request is failing. |
Methods
SendAsync(HttpRequestMessage, CancellationToken)
Sends an HTTP request to the inner handler to send to the server as an asynchronous operation. Retries the request up to the specified maximum retries when encountering a response with the status code 429.
Declaration
protected override async Task<HttpResponseMessage> SendAsync(HttpRequestMessage request, CancellationToken cancellationToken)
Parameters
|
HttpRequestMessage
request
The HTTP request message to send to the server. |
|
System.Threading.CancellationToken
cancellationToken
A cancellation token to cancel operation. |
Returns
|
System.Threading.Tasks.Task<HttpResponseMessage>
Returns System.Threading.Tasks.Task<TResult>. The task object representing the asynchronous operation. |
Exceptions
|
System.ArgumentNullException
The |


Motivation
Geoportals are great platforms to show and give access to available spatial data to the users: all the topics are in one place and you can preview the data before you start downloading them. However, the visualization of the spatial data often lacks cartographic quality, because geoportals are focused on their role as data sharing platforms and not on adequate visualizations of spatial data.
Cartography has entered a new era with the advent of Web 2.0, crowdsourcing and open data movement and thus opening the field to a wider audience. Cartography is not any more only in the hands of national and military agencies! A new type, or rather many new types, of cartographers emerged from this new audience. They create and modify maps online by combining various resources and cartographic tools available. But, even though the access to spatial data and tools became much easier and open, the integration of cartographic principles for the visualization and combination of existing spatial data within geoportals trails behind in its transition to Webmapping 2.0.
Thus this work is motivated by improving the symbolization of spatial data on geoportals, not just because one can make pretty maps, but because well-designed and uncluttered data visualization help gathering more insight about the organization of the spatial phenomenon at hand and creating added value on top of the raw data.
Goals
- Provide cartographic functions to improve the visualization of spatial data on geoportals in user maps
- Integrate these functions as tools in an actual geoportal
- Share and open cartographic knowledge through these tools
- Make pretty and useful maps with available open data


Across: 6. Not 3 down to let German gentleman be confused with FOI (3 4) 7. As 12 across endure something of a crazy topic (3 2) 9. Be possessive, on around toward sunset (3) 10. Cast vege spell for betrayers of close struggle (5 4) 12,19,21,22,1dn,15,3dn. 5 down to stand and deliver! (11 3 3 2 7, 3 2 3 5 3 4) 17. Highly rating identity - he's back! (9) Down: 2. see 5dn. 4. As the unrebellious 7 across, embracing 1000 intra-net characters (9) 5,2,14. Vice, liquor, cars, hepatitis, heists, all of them worshipping in music (7 3 7) 8. Rearrange hanger? (6) 11. Buy dim urn, thus consign to boggy grave (4 2 3) 13. Struts his stuff with such funds (6) 14. see 5dn. 16. 50 in the sack not good enough for 12 across! (5) 18. Look back to old music and audio equipment (4) 20. Luckily arranged to 9 across (3)


I am a second year Computer Science major at University of California, Los Angeles, currently seeking a Software Engineering Intern role in the Summer of 2018. I always ensure quality output from my end and undertake every task with zeal and sincerity.
I am passionate about creating technological products for users and chase this passion by competing at hackathons, where I build some cool things. In the summer of 2017 I worked at OpsVeda, a Silicon Valley startup, and created a prototype blockchain solution for the Global Brands Group.
When I’m away from computers, I enjoy debating and reading about global politics. I take part in Model UN and was the Director of IAEA, BruinMUN 2016. In my free time, I love working out and playing basketball with friends.
In 2016, I graduated from DPS-Gurgaon, India with a Gold Medal in Continuous Academic Excellence. I held the post of Vice Head Boy in the Student Council and Under Secretary in the Model UN Society. I was Founder & President at a community outreach club in school that still impacts lives of many underprivileged students.
A web-platform, hosted on Azure, which made travel safer for Detroit residents. Visualized past crime data, created an algorithm to plot variable 'safe' routes for users using Google Maps API. Enabled in-app interaction with Facebook friends to make “travel buddies”.
Created a color sensing robot that sorted a random arrangement of colored blocks and place them on the respective colored platforms. Programmed the robotic components using LabVIEW, which included line sensing,
gyros and the robotic arm. Won the New Delhi Regional Championship and ranked 4/50 in Nationals.
Created a web-platform, hosted on Azure, which made travel safer for Detroit residents. Visualized past crime data, created an algorithm to plot variable 'safe' routes for users using Google Maps API. Enabled in-app interaction with Facebook friends to make “travel buddies”. Winner of 'Best Local Hack' award at Mhacks 8.
Created a color sensing robot that sorted a random arrangement of colored blocks and place them on the respective colored platforms. Programmed the robotic components using LabVIEW, which included line sensing,
gyros and the robotic arm. Won the New Delhi Regional Championship and ranked 4/50 in Nationals.
A web-platform, hosted on Azure, which made travel safer for Detroit residents. Visualized past crime data, created an algorithm to plot variable 'safe' routes for users using Google Maps API. Enabled in-app interaction with Facebook friends to make “travel buddies”.
Created a color sensing robot that sorted a random arrangement of colored blocks and place them on the respective colored platforms. Programmed the robotic components using LabVIEW, which included line sensing,
gyros and the robotic arm. Won the New Delhi Regional Championship and ranked 4/50 in Nationals.


Learn Bootstrap tutorial for beginners with there easy components and utility.
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Front-end Developer, Designer
4.5Instructor Rating
42 Courses1,10,124 Students
I start my development and digital career studying digital design. After taking a couple of programming classes I realize that code is what I wanted to be doing.
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4.5Instructor Rating
8 Courses12,124 Students
Writing JS, CSS, and HTML professionally since 2004. Open source contributor to projects like jQuery, jQueryUI, ESLint, Webpack, npm, JSCS, Esprima and more.
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4.5 Instructor Rating
23 Courses23,000 Students
Software developer and active open sorcerer. She speaks multiple languages and is often overheard saying "Bonjour" in HTML.
View DetailsFront-end Developer, Designer
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22 Courses40,224 Students
Bree Head developer, architect, occasional designer, and frequent speaker. He’s passionate about building tools, systems, and create high-performance teams and apps.
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--- title: "Yt::Group" h2: "Groups" ---
Yt::Group
represents a YouTube Analytics group.
Initialize using its YouTube ID:
{% include doc.html instance="Group#initialize" %}{% include example.html object='group = Yt::Group' method='new' params=' id: "ABVSo3DwAAA"' %}
{% include example.html result='#<Yt::Group @id=ABVSo3DwAAA>' %}
{% include doc.html instance="Group#canonical_url" %}{% include example.html object='group' method='title' %}
{% include example.html result='"Video group"' %}
All methods of Yt::Group
act on behalf of YouTube accounts (e.g.: to fetch a group).
To use these methods (marked with below), you need to get an API Client ID/Secret from Google, then obtain a refresh token from the account you want to act as, and finally configure the values:
Yt::Group
data methods
{% include doc.html instance="Group#id" %}{% include example.html object='group' method='id' result='"ABVSo3DwAAA"' %}
{% include doc.html instance="Group#title" %}{% include example.html object='group' method='title' result='"Video Group"' %}
{% include doc.html instance="Group#published_at" %}{% include example.html object='group' method='published_at' result='2014-05-02 20:12:57 UTC' %}
{% include doc.html instance="Group#item_count" %}{% include example.html object='group' method='item_count' result='4' %}
{% include doc.html instance="Group#item_type" %}{% include example.html object='group' method='item_type' result='"youtube#video"' %}


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Click on the Reset button above to clean up the grid and restart the game.


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Redirect Set cookie and redirect
This is your DeviceLab redirect page. It only appears if you haven't set a cookie for this page yet, and if you are not forcing it to display by adding '?wait' to the URL.
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Refresh {% endif %}


In Project Talia at Microsoft Research Cambridge, we are exploring how we can best leverage AI to help improve the effectiveness of important mental health services. Specifically, in partnership with SilverCloud Health, we develop machine learning tools to enhance digital mental health services that deliver cognitive-behavioral treatment (CBT) programs to a large and growing number of people in need of effective care.
A key impediment to reinforcement learning (RL) in real applications with limited, batch data is in defining a reward function that reflects what we implicitly know about reasonable behaviour for a task and allows for robust off-policy evaluation. In this work, we develop a method to identify an admissible set of reward functions for policies that (a) do not deviate too far in performance from prior behaviour, and (b) can be evaluated with high confidence, given only a collection of past trajectories. Together, these ensure that we avoid proposing unreasonable policies in high-risk settings.
Applying machine learning tools to forecasting adverse events in intensive care can be invaluable in providing clinicians with the time needed to intervene and improve patient outcomes. In this work, we describe an end-to-end approach to the prediction of hypotension from critical care data using off-the-shelf classification models. Evaluated with standard performance metrics, these models appear to learn effectively from available data, with additional multi-modal information improving classification accuracy. However, we show that this improvement is thrown into question when probing further into medical context and choices in data curation, highlighting the need for domain-centric evaluation.
Laboratory testing is an integral tool in the management of patient care in hospitals, particularly in intensive care units (ICUs). There exists an inherent trade-off in the selection and timing of lab tests between considerations of the expected utility in clinical decision-making of a given test at a specific time, and the associated cost or risk it poses to the patient. To this end, we develop and extend principles of Pareto optimality to improve the selection of actions based on multiple reward function components while respecting typical procedural considerations and prioritization of clinical goals in the ICU. Our experiments show that we can estimate a policy that reduces the frequency of lab tests and optimizes timing to minimize information redundancy.
The management of invasive mechanical ventilation, and the regulation of sedation and analgesia during ventilation, constitutes a major part of the care of patients admitted to intensive care units, but clinical opinion on the best protocol for weaning patients off of a ventilator varies. This work aims to develop a decision support tool that uses available patient information to predict time-to-extubation readiness and to recommend a personalized regime of sedation dosage and ventilator support. To this end, we use off-policy reinforcement learning algorithms to determine the best action at a given patient state from sub-optimal historical ICU data.
Pixie, a socialbot submitted to the 2017 Alexa Prize, was developed with the objective of holding engaging casual conversations on open-ended topics. We develop a modular, heterogeneous architecture, and highlight the successes and shortcomings of our primarily template-based approach. We reflect on our findings on alternative approaches attempted in the prototyping phase, in particular the challenges of bridging breakthroughs in deep learning with the frontier of machine social dialogue.
We consider a dataset of 2-photon excitation microscopy image sequences recorded through a cranial window in GCaMP transgenic mice, showing V1 neuronal activation patterns over time (through increased GFP fluorescence). Analysis of this data requires distinguishing neurons in the image focal plane from other objects, e.g. blood vessels, dendritic branches, and neurons fluorescing above and below the focal plane. The task of neuron identification and boundary detection is currently performed manually but is potentially amenable to automation through various machine learning approaches, such as SVMs or convolutional neural nets. We find that both approaches yield comparable results, and perform well enough to considerably speed up neuron labelling.
This work investigates the scaling properties of Recurrent Neural Network Language Models (RNNLMs) We discuss how to train very large RNNs on GPUs and address the questions of how RNNLMs scale with respect to model size, training-set size, computational costs and memory. We train the largest known RNNs and present relative word error rates gains of 18% on an ASR task. We also present the new lowest perplexities on the recently released billion word language modelling benchmark, 1 BLEU point gain on machine translation and a 17% relative hit rate gain in word prediction.
This project involves the design and development of an adaptive speaker recognition algorithm, tailored for implementation in the Nao domestic service robot for the Chinese market. The problem is fundamentally text independent open set identification; the proposed algorithm distinguishes between a small set of known speakers, and identifies a new speaker as unknown. It is designed to operate in real time, be robust to both background disturbances and internal robot noise, and adapt to changes in the voices of the registered speakers.


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The Government of Man We will now turn our attention a little to the government of man, and see how that will compare with the foregoing, for man stands at the head of this beautiful creation; he is endued with intelligence and capacity for improvement; he is placed as a moral agent, and has the materials put into his hands to work with, the works of his Father as a pattern, the conduct of many of the inferior creation as an example--and might make the earth a garden, a paradise, a place of uninterrupted happiness and felicity, a heaven below. And if God had not delegated this moral agency and power to man, and thus given him the privilege, in part, of being the arbiter of his own destiny, such it would have been to this day, like the Eden from which he was ejected because of his transgression. For he had everything placed within his power, and was made lord of the creation. The beasts, birds, fish, and fowl, were placed under his control; the earth yielded plenty for his wants, and abounded in fruits, grain, herbs, flowers and trees, both to satisfy his hunger, and to please the sight, taste, and smell. The fields waved with plenty, and produced a perennial harvest. The fruits teemed forth in all their luscious varieties to satisfy his most capacious desires. The flowers, in all their gaiety, beauty, and richness, delighted the eye; while their rich fragrance filled the air with odoriferous perfumes. The feathered tribes, with all their gorgeous plumage and variety of song, both pleased the eye, and enchanted and charmed the ear. The horse, the cow, and other animals, were there to promote his happiness, supply his wants, and make him comfortable and happy. All were under his control, to contribute to his happiness and comfort, supply his most extended desires, and to add to his enjoyment; but with all these privileges what is his situation? With celestial blessings within his reach, he has plunged down to the very verge of hell, and is found in a state of poverty, confusion, and distress. He found the earth an Eden--a paradise; he has filled it with misery and woe, and has made it comparatively a howling wilderness. And let us not blame Adam alone for this state of things; for after his ejection from Paradise, the earth was sufficiently fertile to satisfy all the desires of man with moderate industry, and is at the present day, if it were not for the confusion that exists, and if men were properly situated, and its resources developed. But more of this anon. At present we will examine some of these evils, and then point out their cause, and the remedy. We find the world split up and divided into different nations, having different interests, and different objects; with their religious and political views as dissimilar as light and darkness, all the time jealous of each other, and watching each other as so many thieves; and that man at the present day (and it has been the case for ages), is considered the greatest statesman, who, with legislation or diplomacy, can make the most advantageous arrangement with, or coerce by circumstances, other nations into measures that would be for the benefit of the nation with which he is associated. No matter how injurious it might be to the nation or nations concerned, the measure that would yield his nation an advantage, might plunge another in irremediable misery, while there is no one to act as father and parent of the whole, and God is lost sight of. What is it that the private ambition of man has not done to satisfy his craving desires for the acquisition of territory and wealth, and what is falsely called honor and fame? Those private, jarring interests have kept the world in one continual ferment and commotion from the commencement until the present time; and the history of the world is a history of the rise and fall of nations--of wars, commotions, and bloodshed--of nations depopulated, and cities laid waste. Carnage, destruction, and death, have stalked through the earth, exhibiting their horrible forms in all their cadaverous shapes, as though they were the only rightful possessors. Deadly jealousy, fiendish hate, mortal combat, and dying groans, have filled the earth, and our bulwarks, our chronicles, our histories, all bear testimony to this; and even our most splendid paintings, engravings, and statuary, are living memorials of bloodshed, carnage, and destruction. Instead of men being honoured who have sought to promote the happiness, peace, and wellbeing of the human family, and greatness concentrating in that, those have been generally esteemed the most who produced the most misery and distress, and were wholesale robbers, ravagers, and murderers. And from whence come these things? Let the apostle James answer: "..." XXSCRIP. Here is evidently a lack of that consummate wisdom, that moral and physical control, that parental power which balances the universe, and directs the various planets. For let the same recklessness, selfishness, individuality, and nationality there be manifested, and we should see the wildest confusion. Man has come in contact with man, morally, physically, religiously, and nationally, from the foundation of the earth. If God's works had done so, what tumult and ruin there would have been in the immensity of space! Instead of the order that now prevails, man would have been sometimes frozen to death, and at other times burned up; one or two seasons of irregularity, even in climate, would depopulate the earth. But what if the planets, irrespective of the power by which they are controlled, were to rush wildly through space, and, with their mighty impetus dash against each other? "..." There would be "..." What terrible destruction and ruin! We have read of earthquakes destroying countries, of wars depopulating nations--of volcanoes overwhelming cities, and of empires in ruin; but what would the yawning earthquake, the bellowing volcano, the clang of arms, or a nation's distress, be in comparison to a scene like this? System would be shattered with system; planet madly rush on planet; worlds, with their inhabitants, would be destroyed, and creations crumble into ruins. There would be truly a war of planets, "..." These, indeed, would be fearful results, and shew plainly the distinction between the beautiful order of God's work, and the confusion and disorder of man's. God's work is perfect--man's imperfect. The one is the government of God, and the other that of man. We notice the same mismanagement in the arrangement of cities and nations. We have large cities containing immense numbers of human beings, pent up, as it were in one great prison-house, inhaling a foetid, unwholesome atmosphere, impregnated with a thousand deadly poisons; millions of whom, in damp cellars, lonely garrets, and pent up corners, drag out a miserable existence, and their wan faces, haggard countenances, and looks tell but too plainly the tale of their misery and wretchedness. A degenerate, sickly, puny race tread in their steps, inheriting their fathers' misery and distress. If we notice the situation of the nations of Europe at the present time, we see the land burthened with an overplus population, and groaning beneath its inhabitants, while the greatest industry, perseverance, economy, and care, do not suffice to provide for the craving wants of nature. And so fearfully does this prevail in many parts, that parents are afraid to fulfil the first great law of God, "..." and by desperate circumstances are almost forced to the unnatural wish of not propagating their species; while, corrupted with a correspondent depravity with that which reigns among nations, they are found using suicidal measures to prevent an otherwise numerous progeny from increasing their father's misery, and inheriting his misfortunes. And yet, while this is the case, there are immense districts of rich soil, covering millions of square miles, inhabited only by a few untutored savages, or the wild beast of the forest; and such is the infatuation of man that in many districts of country, which were once the seats of the most powerful empires, and where flourished the mightiest nations, there is nothing but desolation and wildness. Such are Nineveh and Babylon, on the Asiatic Continent; and Otolum, and many others discovered by Stephens and Catherwood, in Central America; and recently discovered ruins--unequalled in the old world--a little above the head of the California Gulf. Not only their cities, but their lands are desolate, deserted, and forsaken, and the same evils that once existed there are transferred to another soil, all bespeaking plainly that we want a great, governing, ruling principle to regulate the affairs of the world, and assist poor, feeble, erring humanity. Again, if we examine some of the details of these evils, we shall see more clearly the importance and necessity of a change. Nearly one-third, speaking in general terms, of the inhabitants of the earth are engaged in a calling that would be entirely useless if the world were set right. If men and nations, instead of being governed by their unruly passions, covetous desires, and ambitious motives, were governed by the pure principles of philanthropy, virtue, purity, justice, and honor, and were under the guidance of a fatherly and intelligent head, directed by that wisdom which governs the universe, and regulates the motions of the planetary systems, there would be no need of so many armies, navies, and police regulations, which are now necessary for the protection of those several nations from the aggressions of each other, and internal factions. Let any one examine the position of Europe alone, and he will find this statement abundantly verified. Look at the armies and navies of France and England; and the confusion of Germany, also of Austria, Turkey, Russia and Spain, not to mention many of the smaller nations, and let their armies, their navies, and police be gathered together, and what an abundant host of persons there would be. They would be sufficient to make one of the largest nations in the world! And what are they doing? To use the mildest term, watching each other, as a person would watch a thief for fear of being imposed upon, and robbed, or killed; but generally strolling around as the world's banditti, robbing, plundering, and committing aggressions upon each other; and if they have peace, acquiring it by the sword; and if prevented from aggression and war, it is generally, not that they are governed by just, or virtuous principles, but because they are afraid that aggression might lead to combinations against them which would result in their overthrow and ruin. In the city of Paris alone, at the present time, and its immediate environs, there are one hundred thousand soldiers, besides police to a very great number, not to mention the vast number of custom-house officers and others. Suppose we add to these their families, where they have any, and where they have not, notice the vast amount of prostitution, misery, degradation, and infamy, that such an unnatural state of things produces. I give the above as an example of the whole, but here the navies are not included. I say again, What are these all doing? They do not raise corn to supply the wants of men, nor are they occupied in any useful avocation; but they must live, and their wants must be supplied by the products of the labor of others. There has to be an immense amount of legislation for the accomplishment of this thing, and instead of having one government of righteousness and the world obeying, we have scores of governments, all having to be sustained in regal pomp, to be equal to their neighboring nations; and all this magnificence and national pride having to be supported by the labor of the people. Again, all these legislatures have to provide immense hosts of men, in the shape of custom-house, excise, and police officers, to carry out their designs, all of whom, and their families, help to increase the burden, till it becomes insupportable. That, together with the unnatural state of society, before referred to, in regard to the situation of the inhabitants of cities and the nations, plunges millions of the human family into a state of hopeless destitution, misery, and ruin, for they are groaning under all these hopeless burdens without having sufficient land to till to meet their demands, and as natural means fail they are obliged to have recourse to those that are unnatural. Hence, in England a great majority of the inhabitants are made slaves of, virtually to supply the wants of the greatest part of the world, and are forced to be their laborers. Thousands of them are immured in immense factories, little less than prisons, groaning under a wearisome, sickening, unhealthy labor; deprived of free, wholesome air; weak and emaciated, not having a sufficiency of the necessaries of life. Thousands more from morning till night are immured in pits, shut out from the light of day, the carol of the birds, and the beauty of nature, sickly and weak, in many instances for want of food; and yet, in the midst of their wretchedness, gloom, and misery, you will sometimes hear them trying to sing in their dungeons and prison-houses, in broken, dying accents, "..."
 One of the proprietors informed me that they employed fifteen thousand persons, and paid them £XXNUMBER,XXNUMBER per week. Most of these people labored under ground, in the pits, digging for iron ore and coal; the remainder were employed principally about the furnaces, in rolling the iron, etc., at heavy, laborious, fatiguing work. And who were they toiling for? Principally for the Americans and Russians, at that time, to furnish them with railroad iron. And what did they get for their labor? The riches of those countries? No. £XXNUMBER,XXNUMBER a week among about fifteen thousand persons. I suppose, however, a number of these were boys and girls. The average wages of men was from ten to twelve shillings per week. And this is their pay for that labor; and yet the masters are not to be blamed, that I can learn, for they are forced by competition to this state of things, and by the unnatural, artificial state of society. If they did not do this their workmen must be out of employ, and ten times worse off, if that were possible, than they are now. In the State of XXSTATE, in America, where the railroads run through coal and iron mines both, they leave them untouched, and come to England for iron to make the rails of, that they cannot afford to make at home, because of higher wages, and an outlet to society, which prevents them from being coerced into bondage.  When nations and rulers set the pattern, they generally find plenty to follow their example; hence covetousness, fraud, rapine, bloodshed, and murder, prevail to an alarming extent. If a nation is covetous, an individual thinks he may be also; if a nation commits a fraud, it sanctions his acts in a small way; and if a nation engages in wholesale robbery, an individual does not see the impropriety of doing it in retail; if a strong nation oppresses a weak one, he does not see why he may not have the same privilege; corruption follows corruption, and fraud treads on the heels of fraud, and all those noble, honourable, virtuous, principles that ought to govern men are lost sight of, and chicanery and deception ride rampant through the world. The welfare, happiness, exaltation, and glory of man, are sacrificed at the shrine of ambition, pride, covetousness and lasciviousness. By these means nations are overthrown, kingdoms destroyed, communities broken up, families rendered miserable, and individuals ruined. I might enter into a detail of the crimes, abominations, lusts, and corruptions that exist in many of our large cities, but I shall leave this subject, and conclude with the remarks of the prophet XXPROPHET, who gazed in prophetic vision on this scene: "..."
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Those great national evils of which I have spoken are things which at present seem to be out of the reach of human agency, legislation, or control. They are diseases that have been generating for centuries; that have entered into the vitals of all institutions, religious and political; that have prostrated the powers and energies of all bodies politic, and left the world to groan under them, for they are evils that exist in church and state, at home and abroad; among Jew and Gentile, Christian, Pagan, and Mahomedan; king, prince, courtier, and peasant; like the deadly simoon, they have paralyzed the energies, broken the spirits, damped the enterprize, corrupted the morals, and crushed the hopes of the world.
thoseXXgreatXXnationalXXevils greatXXnationalXXevilsXXof nationalXXevilsXXofXXwhich evilsXXofXXwhichXXi ofXXwhichXXiXXhave whichXXiXXhaveXXspoken iXXhaveXXspokenXXare haveXXspokenXXareXXthings spokenXXareXXthingsXXwhich areXXthingsXXwhichXXat thingsXXwhichXXatXXpresent whichXXatXXpresentXXseem atXXpresentXXseemXXto presentXXseemXXtoXXbe seemXXtoXXbeXXout toXXbeXXoutXXof beXXoutXXofXXthe outXXofXXtheXXreach ofXXtheXXreachXXof theXXreachXXofXXhuman reachXXofXXhumanXXagency ofXXhumanXXagencyXXlegislation humanXXagencyXXlegislationXXor agencyXXlegislationXXorXXcontrol legislationXXorXXcontrolXXthey orXXcontrolXXtheyXXare controlXXtheyXXareXXdiseases theyXXareXXdiseasesXXthat areXXdiseasesXXthatXXhave diseasesXXthatXXhaveXXbeen thatXXhaveXXbeenXXgenerating haveXXbeenXXgeneratingXXfor beenXXgeneratingXXforXXcenturies generatingXXforXXcenturiesXXthat forXXcenturiesXXthatXXhave centuriesXXthatXXhaveXXentered thatXXhaveXXenteredXXinto haveXXenteredXXintoXXthe enteredXXintoXXtheXXvitals intoXXtheXXvitalsXXof theXXvitalsXXofXXall vitalsXXofXXallXXinstitutions ofXXallXXinstitutionsXXreligious allXXinstitutionsXXreligiousXXand institutionsXXreligiousXXandXXpolitical religiousXXandXXpoliticalXXthat andXXpoliticalXXthatXXhave politicalXXthatXXhaveXXprostrated thatXXhaveXXprostratedXXthe haveXXprostratedXXtheXXpowers prostratedXXtheXXpowersXXand theXXpowersXXandXXenergies powersXXandXXenergiesXXof andXXenergiesXXofXXall energiesXXofXXallXXbodies ofXXallXXbodiesXXpolitic allXXbodiesXXpoliticXXand bodiesXXpoliticXXandXXleft politicXXandXXleftXXthe andXXleftXXtheXXworld leftXXtheXXworldXXto theXXworldXXtoXXgroan worldXXtoXXgroanXXunder toXXgroanXXunderXXthem groanXXunderXXthemXXfor underXXthemXXforXXthey themXXforXXtheyXXare forXXtheyXXareXXevils theyXXareXXevilsXXthat areXXevilsXXthatXXexist evilsXXthatXXexistXXin thatXXexistXXinXXchurch existXXinXXchurchXXand inXXchurchXXandXXstate churchXXandXXstateXXat andXXstateXXatXXhome stateXXatXXhomeXXand atXXhomeXXandXXabroad homeXXandXXabroadXXamong andXXabroadXXamongXXjew abroadXXamongXXjewXXand amongXXjewXXandXXgentile jewXXandXXgentileXXchristian andXXgentileXXchristianXXpagan gentileXXchristianXXpaganXXand christianXXpaganXXandXXmahomedan paganXXandXXmahomedanXXking andXXmahomedanXXkingXXprince mahomedanXXkingXXprinceXXcourtier kingXXprinceXXcourtierXXand princeXXcourtierXXandXXpeasant courtierXXandXXpeasantXXlike andXXpeasantXXlikeXXthe peasantXXlikeXXtheXXdeadly likeXXtheXXdeadlyXXsimoon theXXdeadlyXXsimoonXXthey deadlyXXsimoonXXtheyXXhave simoonXXtheyXXhaveXXparalyzed theyXXhaveXXparalyzedXXthe haveXXparalyzedXXtheXXenergies paralyzedXXtheXXenergiesXXbroken theXXenergiesXXbrokenXXthe energiesXXbrokenXXtheXXspirits brokenXXtheXXspiritsXXdamped theXXspiritsXXdampedXXthe spiritsXXdampedXXtheXXenterprize dampedXXtheXXenterprizeXXcorrupted theXXenterprizeXXcorruptedXXthe enterprizeXXcorruptedXXtheXXmorals corruptedXXtheXXmoralsXXand theXXmoralsXXandXXcrushed moralsXXandXXcrushedXXthe andXXcrushedXXtheXXhopes crushedXXtheXXhopesXXof theXXhopesXXofXXthe hopesXXofXXtheXXworld
thoseXXgreatXXnational greatXXnationalXXevils nationalXXevilsXXof evilsXXofXXwhich ofXXwhichXXi whichXXiXXhave iXXhaveXXspoken haveXXspokenXXare spokenXXareXXthings areXXthingsXXwhich thingsXXwhichXXat whichXXatXXpresent atXXpresentXXseem presentXXseemXXto seemXXtoXXbe toXXbeXXout beXXoutXXof outXXofXXthe ofXXtheXXreach theXXreachXXof reachXXofXXhuman ofXXhumanXXagency humanXXagencyXXlegislation agencyXXlegislationXXor legislationXXorXXcontrol orXXcontrolXXthey controlXXtheyXXare theyXXareXXdiseases areXXdiseasesXXthat diseasesXXthatXXhave thatXXhaveXXbeen haveXXbeenXXgenerating beenXXgeneratingXXfor generatingXXforXXcenturies forXXcenturiesXXthat centuriesXXthatXXhave thatXXhaveXXentered haveXXenteredXXinto enteredXXintoXXthe intoXXtheXXvitals theXXvitalsXXof vitalsXXofXXall ofXXallXXinstitutions allXXinstitutionsXXreligious institutionsXXreligiousXXand religiousXXandXXpolitical andXXpoliticalXXthat politicalXXthatXXhave thatXXhaveXXprostrated haveXXprostratedXXthe prostratedXXtheXXpowers theXXpowersXXand powersXXandXXenergies andXXenergiesXXof energiesXXofXXall ofXXallXXbodies allXXbodiesXXpolitic bodiesXXpoliticXXand politicXXandXXleft andXXleftXXthe leftXXtheXXworld theXXworldXXto worldXXtoXXgroan toXXgroanXXunder groanXXunderXXthem underXXthemXXfor themXXforXXthey forXXtheyXXare theyXXareXXevils areXXevilsXXthat evilsXXthatXXexist thatXXexistXXin existXXinXXchurch inXXchurchXXand churchXXandXXstate andXXstateXXat stateXXatXXhome atXXhomeXXand homeXXandXXabroad andXXabroadXXamong abroadXXamongXXjew amongXXjewXXand jewXXandXXgentile andXXgentileXXchristian gentileXXchristianXXpagan christianXXpaganXXand paganXXandXXmahomedan andXXmahomedanXXking mahomedanXXkingXXprince kingXXprinceXXcourtier princeXXcourtierXXand courtierXXandXXpeasant andXXpeasantXXlike peasantXXlikeXXthe likeXXtheXXdeadly theXXdeadlyXXsimoon deadlyXXsimoonXXthey simoonXXtheyXXhave theyXXhaveXXparalyzed haveXXparalyzedXXthe paralyzedXXtheXXenergies theXXenergiesXXbroken energiesXXbrokenXXthe brokenXXtheXXspirits theXXspiritsXXdamped spiritsXXdampedXXthe dampedXXtheXXenterprize theXXenterprizeXXcorrupted enterprizeXXcorruptedXXthe corruptedXXtheXXmorals theXXmoralsXXand moralsXXandXXcrushed andXXcrushedXXthe crushedXXtheXXhopes theXXhopesXXof hopesXXofXXthe ofXXtheXXworld
thoseXXgreat greatXXnational nationalXXevils evilsXXof ofXXwhich whichXXi iXXhave haveXXspoken spokenXXare areXXthings thingsXXwhich whichXXat atXXpresent presentXXseem seemXXto toXXbe beXXout outXXof ofXXthe theXXreach reachXXof ofXXhuman humanXXagency agencyXXlegislation legislationXXor orXXcontrol controlXXthey theyXXare areXXdiseases diseasesXXthat thatXXhave haveXXbeen beenXXgenerating generatingXXfor forXXcenturies centuriesXXthat thatXXhave haveXXentered enteredXXinto intoXXthe theXXvitals vitalsXXof ofXXall allXXinstitutions institutionsXXreligious religiousXXand andXXpolitical politicalXXthat thatXXhave haveXXprostrated prostratedXXthe theXXpowers powersXXand andXXenergies energiesXXof ofXXall allXXbodies bodiesXXpolitic politicXXand andXXleft leftXXthe theXXworld worldXXto toXXgroan groanXXunder underXXthem themXXfor forXXthey theyXXare areXXevils evilsXXthat thatXXexist existXXin inXXchurch churchXXand andXXstate stateXXat atXXhome homeXXand andXXabroad abroadXXamong amongXXjew jewXXand andXXgentile gentileXXchristian christianXXpagan paganXXand andXXmahomedan mahomedanXXking kingXXprince princeXXcourtier courtierXXand andXXpeasant peasantXXlike likeXXthe theXXdeadly deadlyXXsimoon simoonXXthey theyXXhave haveXXparalyzed paralyzedXXthe theXXenergies energiesXXbroken brokenXXthe theXXspirits spiritsXXdamped dampedXXthe theXXenterprize enterprizeXXcorrupted corruptedXXthe theXXmorals moralsXXand andXXcrushed crushedXXthe theXXhopes hopesXXof ofXXthe theXXworld
Thousands of men would desire to do good, if they only knew how; but they see not the foundation and extent of the evil, and long-established opinions, customs and doctrines, blind their eyes, and damp their energies. And if a few should see the evil, and try a remedy, what are a few in opposition to the views, power, influence, and corruption of the world?
thousandsXXofXXmenXXwould ofXXmenXXwouldXXdesire menXXwouldXXdesireXXto wouldXXdesireXXtoXXdo desireXXtoXXdoXXgood toXXdoXXgoodXXif doXXgoodXXifXXthey goodXXifXXtheyXXonly ifXXtheyXXonlyXXknew theyXXonlyXXknewXXhow onlyXXknewXXhowXXbut knewXXhowXXbutXXthey howXXbutXXtheyXXsee butXXtheyXXseeXXnot theyXXseeXXnotXXthe seeXXnotXXtheXXfoundation notXXtheXXfoundationXXand theXXfoundationXXandXXextent foundationXXandXXextentXXof andXXextentXXofXXthe extentXXofXXtheXXevil ofXXtheXXevilXXand theXXevilXXandXXlong evilXXandXXlongXXestablished andXXlongXXestablishedXXopinions longXXestablishedXXopinionsXXcustoms establishedXXopinionsXXcustomsXXand opinionsXXcustomsXXandXXdoctrines customsXXandXXdoctrinesXXblind andXXdoctrinesXXblindXXtheir doctrinesXXblindXXtheirXXeyes blindXXtheirXXeyesXXand theirXXeyesXXandXXdamp eyesXXandXXdampXXtheir andXXdampXXtheirXXenergies dampXXtheirXXenergiesXXand theirXXenergiesXXandXXif energiesXXandXXifXXa andXXifXXaXXfew ifXXaXXfewXXshould aXXfewXXshouldXXsee fewXXshouldXXseeXXthe shouldXXseeXXtheXXevil seeXXtheXXevilXXand theXXevilXXandXXtry evilXXandXXtryXXa andXXtryXXaXXremedy tryXXaXXremedyXXwhat aXXremedyXXwhatXXare remedyXXwhatXXareXXa whatXXareXXaXXfew areXXaXXfewXXin aXXfewXXinXXopposition fewXXinXXoppositionXXto inXXoppositionXXtoXXthe oppositionXXtoXXtheXXviews toXXtheXXviewsXXpower theXXviewsXXpowerXXinfluence viewsXXpowerXXinfluenceXXand powerXXinfluenceXXandXXcorruption influenceXXandXXcorruptionXXof andXXcorruptionXXofXXthe corruptionXXofXXtheXXworld
thousandsXXofXXmen ofXXmenXXwould menXXwouldXXdesire wouldXXdesireXXto desireXXtoXXdo toXXdoXXgood doXXgoodXXif goodXXifXXthey ifXXtheyXXonly theyXXonlyXXknew onlyXXknewXXhow knewXXhowXXbut howXXbutXXthey butXXtheyXXsee theyXXseeXXnot seeXXnotXXthe notXXtheXXfoundation theXXfoundationXXand foundationXXandXXextent andXXextentXXof extentXXofXXthe ofXXtheXXevil theXXevilXXand evilXXandXXlong andXXlongXXestablished longXXestablishedXXopinions establishedXXopinionsXXcustoms opinionsXXcustomsXXand customsXXandXXdoctrines andXXdoctrinesXXblind doctrinesXXblindXXtheir blindXXtheirXXeyes theirXXeyesXXand eyesXXandXXdamp andXXdampXXtheir dampXXtheirXXenergies theirXXenergiesXXand energiesXXandXXif andXXifXXa ifXXaXXfew aXXfewXXshould fewXXshouldXXsee shouldXXseeXXthe seeXXtheXXevil theXXevilXXand evilXXandXXtry andXXtryXXa tryXXaXXremedy aXXremedyXXwhat remedyXXwhatXXare whatXXareXXa areXXaXXfew aXXfewXXin fewXXinXXopposition inXXoppositionXXto oppositionXXtoXXthe toXXtheXXviews theXXviewsXXpower viewsXXpowerXXinfluence powerXXinfluenceXXand influenceXXandXXcorruption andXXcorruptionXXof corruptionXXofXXthe ofXXtheXXworld
thousandsXXof ofXXmen menXXwould wouldXXdesire desireXXto toXXdo doXXgood goodXXif ifXXthey theyXXonly onlyXXknew knewXXhow howXXbut butXXthey theyXXsee seeXXnot notXXthe theXXfoundation foundationXXand andXXextent extentXXof ofXXthe theXXevil evilXXand andXXlong longXXestablished establishedXXopinions opinionsXXcustoms customsXXand andXXdoctrines doctrinesXXblind blindXXtheir theirXXeyes eyesXXand andXXdamp dampXXtheir theirXXenergies energiesXXand andXXif ifXXa aXXfew fewXXshould shouldXXsee seeXXthe theXXevil evilXXand andXXtry tryXXa aXXremedy remedyXXwhat whatXXare areXXa aXXfew fewXXin inXXopposition oppositionXXto toXXthe theXXviews viewsXXpower powerXXinfluence influenceXXand andXXcorruption corruptionXXof ofXXthe theXXworld
No power on this side of heaven can correct the evil. It is a world that is degenerated, and it requires a God to put it right.
noXXpowerXXonXXthis powerXXonXXthisXXside onXXthisXXsideXXof thisXXsideXXofXXheaven sideXXofXXheavenXXcan ofXXheavenXXcanXXcorrect heavenXXcanXXcorrectXXthe canXXcorrectXXtheXXevil correctXXtheXXevilXXit theXXevilXXitXXis evilXXitXXisXXa itXXisXXaXXworld isXXaXXworldXXthat aXXworldXXthatXXis worldXXthatXXisXXdegenerated thatXXisXXdegeneratedXXand isXXdegeneratedXXandXXit degeneratedXXandXXitXXrequires andXXitXXrequiresXXa itXXrequiresXXaXXgod requiresXXaXXgodXXto aXXgodXXtoXXput godXXtoXXputXXit toXXputXXitXXright
noXXpowerXXon powerXXonXXthis onXXthisXXside thisXXsideXXof sideXXofXXheaven ofXXheavenXXcan heavenXXcanXXcorrect canXXcorrectXXthe correctXXtheXXevil theXXevilXXit evilXXitXXis itXXisXXa isXXaXXworld aXXworldXXthat worldXXthatXXis thatXXisXXdegenerated isXXdegeneratedXXand degeneratedXXandXXit andXXitXXrequires itXXrequiresXXa requiresXXaXXgod aXXgodXXto godXXtoXXput toXXputXXit putXXitXXright
noXXpower powerXXon onXXthis thisXXside sideXXof ofXXheaven heavenXXcan canXXcorrect correctXXthe theXXevil evilXXit itXXis isXXa aXXworld worldXXthat thatXXis isXXdegenerated degeneratedXXand andXXit itXXrequires requiresXXa aXXgod godXXto toXXput putXXit itXXright


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abstract fun onCommunicatingStateChanged(isCommunicating: Boolean): Unit
Notification method called when network communication is beginning or ending.
isCommunicating - true if communication is starting, false if it is stopping.
isCommunicating
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I am passionate about designing and developing beautiful, functional and seamless web and mobile applications. I thrive in agile environments and enjoy pair programming as well as working on my own. Researching and usability testing are dear to my heart, as I believe they are the first steps in creating an intuitive experience.
Managed a small business start-up in a niche market by creating original infusions using traditional methods and ingredients.
Essential team member and Managing Fashion Photo Editor at the leading online marketplace for the world’s most beautiful things.
Graphic Designer, Performed Ecommerce Website Maintenance, Managed Email Campaigns, Managed Extensive Database, Assisted Head Merchandiser
Apart from being a web developer, I love to tinker with anything I can get my hands on. I love learning new crafts - from circuit boards to knitting to robotics to a new computer program.
When I'm forced indoors, I love to bake and consider myself a foodie and health-food geek. I follow a number of sci-fi and fantasy genre movies and television shows, and I spend a large amount of my free time exploring the latest technolgy advancements in the front-end web development world. I'm also a novice indoor rock climber.


Due to different work flows, we use different statistical tests to determine if the phenotyping results of a knockout line differ from the wild type mice. The two main approaches we are using are a reference range approach for continuous data and a Fisher’s exact test for categorical data.
Our statistical analysis is designed to differentiate as efficiently as possible between lines that show a phenotype and lines that do not. However, the criteria for calling a hit are very stringent, in order to make sure that when you order a mouse in, the phenotype we have observed will hold up. The other side of the coin is that we are likely to miss true hits, i.e. have a higher number of false negatives. Therefore we encourage you to take a look at the raw data for phenotypes you are interested in and judge for yourself if a borderline case might actually be a true phenotype, even if it hasn't met our very stringent statistical criteria.
For every candidate hit identified in the high-throughput analysis, we went back to the underlying raw data (FCS files for flow cytometry etc) for the candidate strain and appropriate controls and a final call was made by an expert in the field.
All tests from the observational screen are carried out on mice of the main Sanger phenotyping pipeline. An average of 84 mice are phenotyped every week on two separate days. Due to operational reasons, wild type controls are only processed on one of these days. Knockout mice enter the pipeline according to their age and mice from one strain are phenotyped on at least two different days to avoid any day effects. Due to this set-up, knockout mice cannot be compared to controls on the same day. Using daily controls would also not take advantage of the many wild type data points that are collected over time.
Hence we wanted an approach which would:Therefore we decided to use reference range (RR) approach to analyse the data.
Data from at least 70 wild type mice was used to generate a reference range. Knockout lines are considered to be significantly different from wild type mice if 60% of the mice for this strain lie outside the middle 95% of wild type data points, i.e. if they lie above the 97.5th or below the 2.5th percentile of the wild type data. For assays with 2 or 3 mice per gender, separate RRs are constructed for male and female mice, but hits are only called if 60% of samples across both genders are outside their respective reference ranges (3 out of 4 or 4 out of 6). For assays with at least 4 mice per gender (Blood flow cytometry), males and females are assessed fully separately.
For flow cytometry assays of tissues other than blood, we constructed time-window reference range (TRR), by comparing each KO mouse to at least 70 WT of matching sex closest in time and combining relation to TRR (“within”, “above”, “below”) across all KO mouse from a strain.
The reference range approach does not take into account the actual distribution of the knockout data, therefore a p-value cannot be calculated. Assuming that all samples are independent of each other, false positive rates vary between 1 in 4.8 million for 7 mice per gender per knockout line (e.g. 16 week blood) and 1 in 16,000 for 4 mice per knockout line (e.g. for cytotoxicity).
The screen for antinuclear antibodies in serum and the test for worm burden in Trichuris infection have a very different data structure than the other assays, as wild types are usually negative and positivity is a strong indication for an abnormal phenotype. These assays are therefore analysed with the categorical Fisher’s exact test.
Even though the intensity of antinuclear antibody is quantified on a continuous scale, the main readout is the presence or absence of antibody staining. The data is split into negative (scores 0-1) and positive samples (scores 2-5) and analysed categorically. Similarly, worms in the cecum could be counted to assess worm burden, but the more important finding is that worms are present at all.
Cell numbers for each population, expressed as percentage of parent gate, percentage of CD45+ cells.
Sex-specific, time-window reference range (TRR), manual verification of candidate hits
Reference ranges were constructed using the 70 wt mice of the same sex that were closest in time (TRR). Cell subset measurements for all mice are available to download from the “More data” tab. Data and code used for identification of candidate hits are available on github. Final calls were made by an experienced researcher after reanalysis of the data in FlowJo.
The project started in late autumn 2013. In mid-2014, we decided to change the original antibody panels to make them more compatible with the IMPC panels and to replace a few antibodies that had not been working as well as expected. For the T cell panel, this change happened on 9th of June 2014, for the B cell panel on 15th of September 2014. Data collected before and after the panel changes are not directly comparable and were analysed separately (constructing separate reference ranges before and after the panel change).
We were aware at the start of the project that manual gating of all FCS files would be very time-consuming and started immediately to explore different algorithms to automate the gating process. However, quality control of ongoing phenotyping meant that we still had to gate all files manually before the automated gating was in place. We eventually teamed up with Ryan Brinkman’s team in Vancouver. and developed a semi-supervised gating algorithm for all our panels except blood after the initial panel change in summer 2014. Getting this right and performing the required validations took longer than anticipated and we therefore ended up with a complete set of manually gated data and an almost complete set of data gated automatically with a semi-supervised algorithm. Both datasets are available in the data download section.
Data sent to the IMPC for graphing on the website is based on manual gating. For the consortium publication, we reanalysed all available data with the semi-supervised algorithm and refined the hit calling accordingly. Hits displayed online are therefore based on manual gating but verified with automated gating. We found that automated gating reduced temporal drift in many parameters but had not a huge impact on the hit calling as the reference range only calls extreme phenotypes. For further analysis, especially for potential phenotypes that have not made our stringent cut-off criteria, we recommend using the automated data wherever possible.
Automated semi-supervised gating Computational gating was performed by Ryan Brinkman’s team with flowClean, UFO, and flowDensity . FlowClean was used to perform acquisition-based quality checking to remove anomalous events. Files with fewer than 20,000 events were removed from further analysis. UFO was used to identify outlier samples (e.g., batch effects). FlowDensity was used to enumerate cell populations by automating a predefined gating approach using sequential, supervised bivariate gating analysis to set the best cut-off for an individual marker using characteristics of the density distribution. The parameters for each individual cell population were pre-defined once for all files. The automated analysis data was validated against matched manually analysed data.
Cell numbers for each population, expressed as percentage of parent gate and as absolute numbers
Sex-specific reference range, manual verification of candidate hits
Cell subset measurements for all mice are available to download from the “More data” tab. Data and code used for identification of candidate hits are available on github. Final calls were made by an experienced researcher after reanalysis of the data in FlowJo.
The blood data was analysed by us and by the IMPC. The reference range approach has been used extensively by WTSI and 3i, but is currently not available as a test in the IMPC statistical pipeline. The IMPC has therefore analysed the same blood data using a mixed model approach which is less stringent and leads to more hits. Hits in blood immunophenotyping will therefore differ between this webpage and the IMPC webpage. Our approach is more conservative and very unlikely to contain false positive results. The IMPC analysis offers an assessment of which genes score a hit with a less stringent statistical approach.
Number of cells per field of view, percentage of interacting cells, cell volume, cell shape (roundness), number and length of dendrites
Reference range, manual verification of candidate strains Measurements for all mice are available to download from the “More data” tab. Data and code used for identification of candidate hits are available on github. Final calls were made by an experienced researcher after reinspection of the raw and processed data in Definiens Developer.
Cytotoxicity is expressed as percent killing in comparison to the positive control, data is generated for five different effector:target ratios
Reference range
Since the assay generates a curve consisting of different target:effector ratios rather than a single value, a curve is considered to be outside the reference range if three consecutive values lie outside their reference range interval. Data for all mice is available to download from the “More data” tab.
Scores corresponding to intensity of antibody staining
Fisher’s exact test
Approximately 10% of wild type mice show a positive staining for antinuclear antibodies. Against this background, 4 out of 6 samples for a given knockout line need to be positive to reach a p-value below 0.01. Data for all mice is available to download from the “More data” tab.
Weight loss: Percentage of reduction in body weight compared to the start of the experiment, from daily weight measurements Survival: Number of mice having to be sacrificed because of excessive weight loss before the end of the experiment
Mixed model with concurrent wt controls for weight loss, Fisher’s exact test for the number of sacrificed mice
Weight loss hits were identified by fitting a robust mixed model (corresponding to two-way Anova) with a p-value cut-off of 0.0001, in comparison to concurrent wt controls, taking into account sex and genotype, using the R package Phenstat. Residuals were assumed to be normally distributed. Calls were made when a genotype has a significant main effect on weight loss.
Colony forming units (CFU) of bacteria cultured on LB agar isolated from spleen and liver homogenates, titers of antigen-specific antibodies in serum measured by ELISA
Mann-Whitney-U test
Hits were identified by a Mann-Whitney-U test with a p-value cut-off of 0.05 in comparison to concurrent WT controls. The analysis combined data from male and female mice. Significant lines were retested with a second cohort. A final call was made on the combined data from both experiments.
Presence or absence of worms in the cecum as categorical measure
Fisher’s exact test
Wild type mice clear a Trichuris muris infection completely. Presence of worms in the cecum at day 33 therefore indicates a higher susceptibility. Approximately 10% of wild type mice have a low number of worms in the cecum at day 33. Against this background, 4 out of 6 samples for a given knockout line need to be positive to reach a p-value below 0.01. Data for all mice is available to download from the “More data” tab.
Histology scores: Histology sections from mid and distal colon are assessed in four different categories indicating damage to the gut. Categories are scored individually (each on scale 0-3) and combined as a total score. Weight loss: Percentage of reduction in body weight compared to the start of the experiment, from the daily weight measurements. Survival: Number of mice having to be sacrificed because of excessive weight loss before the end of the experiment.
Weight loss: Percentage of reduction in body weight compared to the start of the experiment, from the daily weight measurements.
Survival: Number of mice having to be culled because of excessive weight loss before the end of the experiment.
Weight loss/ histology scores: sex-matched reference range, 70 individuals closest in starting weight (histology) or age (weight loss).
Survival: Fisher’s exact test with a p-value cut-off of 0.01, comparing the frequency of sacrificed mice in a mutant strain with the wt population, combining data from both sexes.
Measurements for all mice are available to download from the “More data” tab. Data and code used for identification of candidate hits are available on github. Final calls were made by an experienced researcher after reinspection of the raw and processed data.


public class RootEditPart extends org.eclipse.gef.editparts.ScalableFreeformRootEditPart implements ZoomableEditPart
CONNECTION_LAYER, FEEDBACK_LAYER, GRID_LAYER, GUIDE_LAYER, HANDLE_LAYER, PRIMARY_LAYER, PRINTABLE_LAYERS, SCALABLE_LAYERS, SCALED_FEEDBACK_LAYER
REQ_ADD, REQ_ALIGN, REQ_ALIGN_CHILDREN, REQ_CLONE, REQ_CONNECTION_END, REQ_CONNECTION_START, REQ_CREATE, REQ_CREATE_BENDPOINT, REQ_DELETE, REQ_DELETE_DEPENDANT, REQ_DIRECT_EDIT, REQ_MOVE, REQ_MOVE_BENDPOINT, REQ_MOVE_CHILDREN, REQ_OPEN, REQ_ORPHAN, REQ_ORPHAN_CHILDREN, REQ_RECONNECT_SOURCE, REQ_RECONNECT_TARGET, REQ_RESIZE, REQ_RESIZE_CHILDREN, REQ_SELECTION, REQ_SELECTION_HOVER
| Constructor and Description |
|---|
RootEditPart() |
| Modifier and Type | Method and Description |
|---|---|
org.eclipse.draw2d.LayeredPane |
getPrintableLayers() |
org.eclipse.gef.editparts.ZoomManager |
getZoomManager() |
void |
preferenceUpdate()
Preference update.
|
void |
zoomIn()
zoomIn Method will zoom in the EditPart to the next available level.
|
void |
zoomIn(org.eclipse.draw2d.geometry.Point center)
zoomIn Method will zoom in the EditPart to the next available level
centered around a given point.
|
void |
zoomOut()
zoomOut Method will zoom out the EditPart to the next available level.
|
void |
zoomOut(org.eclipse.draw2d.geometry.Point center)
zoomOut Method will zoom out the EditPart to the next available level
centered around a given point.
|
void |
zoomTo(double zoom,
org.eclipse.draw2d.geometry.Point center)
zoomTo This method allows implementators to zoom to a certain level
centered around a given point.
|
void |
zoomTo(org.eclipse.draw2d.geometry.Rectangle rect)
zoomTo This method allows implementors to zoom into or out to a
rectangular area.
|
getAdapter, getContentPane, getDragTracker, getModel
getCommand, getContents, getRoot, getViewer, setContents, setViewer
activate, addNodeListener, addNotify, deactivate, getFigure, getSourceConnections, getTargetConnections, refresh, removeNodeListener, removeNotify, setLayoutConstraint
addEditPartListener, eraseSourceFeedback, eraseTargetFeedback, getChildren, getEditPolicy, getParent, getSelected, getTargetEditPart, hasFocus, installEditPolicy, isActive, isSelectable, performRequest, removeEditPartListener, removeEditPolicy, setFocus, setModel, setParent, setSelected, showSourceFeedback, showTargetFeedback, toString, understandsRequest
equals, getClass, hashCode, notify, notifyAll, wait, wait, wait
activate, addEditPartListener, addNotify, deactivate, eraseSourceFeedback, eraseTargetFeedback, getChildren, getEditPolicy, getParent, getSelected, getTargetEditPart, hasFocus, installEditPolicy, isActive, isSelectable, performRequest, refresh, removeEditPartListener, removeEditPolicy, removeNotify, setFocus, setModel, setParent, setSelected, showSourceFeedback, showTargetFeedback, understandsRequest
public org.eclipse.draw2d.LayeredPane getPrintableLayers()
getPrintableLayers
in class org.eclipse.gef.editparts.FreeformGraphicalRootEditPart
public org.eclipse.gef.editparts.ZoomManager getZoomManager()
getZoomManager
in class org.eclipse.gef.editparts.ScalableFreeformRootEditPart
public void preferenceUpdate()
public void zoomTo(double zoom, org.eclipse.draw2d.geometry.Point center)
ZoomableEditPart
zoomTo
in interface ZoomableEditPart
zoom
- double value where 1.0 represents 100%.center
- Point around which the zoom will be centered in absolute
coordinatespublic void zoomTo(org.eclipse.draw2d.geometry.Rectangle rect)
ZoomableEditPart
zoomTo
in interface ZoomableEditPart
rect
- Rectangle that the edit part will zoom into our out to in
absolute coordinates.public void zoomIn()
ZoomableEditPart
zoomIn
in interface ZoomableEditPart
public void zoomIn(org.eclipse.draw2d.geometry.Point center)
ZoomableEditPart
zoomIn
in interface ZoomableEditPart
center
- Point around which the zoom will be centered in absolute
coordinatespublic void zoomOut()
ZoomableEditPart
zoomOut
in interface ZoomableEditPart
public void zoomOut(org.eclipse.draw2d.geometry.Point center)
ZoomableEditPart
zoomOut
in interface ZoomableEditPart
center
- Point around which the zoom will be centered in absolute
coordinates


×
120 SECONDS
DESCRIPTION
Wanna be a director? Cinematographer? An actor? We are waiting for your stack of shorts.Start, Camera, Action.
RULES
- Maximum run time 120 seconds(Including end credits and post credits, if any.
- The entries should be submitted on or before 26-3-19 9:30 AM.
- Malayalam or English language can be used in the film. Use of other languages will not be entertained.
- Decision made by the committee will be final.


Reds outright Derek Dietrich to Triple-A
Colón, Mella, Stephens also cleared waivers and were sent to Louisvile
by Mark SheldonCINCINNATI -- With big home runs and several clutch moments, infielder/outfielder Derek Dietrich was one of the Reds’ most popular players of the first half in 2019. But on Monday, Dietrich was one of four players taken off Cincinnati’s 40-man roster.
Dietrich, along with infielder Christian Colón and right-handers Keury Mella and Jackson Stephens, cleared waivers and were sent outright to Triple-A Louisville.
The Reds’ 40-man roster now stands at 35 players.
In 113 games for the Reds, Dietrich batted .187/.328/.462 with 19 home runs and 43 RBIs. Despite only 67 starts, he tied for third on the club in homers. Dietrich had signed a Minor League contract in February, with a deal worth $2 million after making the team.
Dietrich, 30, hit 18 of his homers before the All-Star break and gained immediate popularity among fans and media. He became the fourth Reds player to belt 10 homers before reaching 100 plate appearances, and the first since Tony Perez in 1970. On May 28 against the Pirates, Dietrich knocked three home runs with six RBIs.
That was all before Dietrich endured hard times at the plate the rest of the season, batting .071 with one homer in the second half.
Among the others taken off the roster, Mella ranked as the organization’s No. 23 prospect per MLB Pipeline. The Reds acquired him along with outfielder Adam Duvall from the Giants in the 2015 Trade Deadline deal for right-hander Mike Leake. In eight big league outings over three seasons, Mella posted a 7.94 ERA. He went 8-14 with a 5.05 ERA in 27 starts this season for Louisville.
Mark Sheldon has covered the Reds for MLB.com since 2006, and previously covered the Twins from 2001-05. Follow him on Twitter @m_sheldon and Facebook.


trueChart-Menubar: comprehensive navigational options, (pre-)selection and other trigger-based actions
Online Manual


Fouaad Mirza Seals Historic Olympic Qualification
Fouaad Mirza on Friday became the first Indian equestrian in 20 years to seal an Olympic quota.
by Indo-Asian News ServiceFouaad Mirza on Friday became the first Indian equestrian in 20 years to seal an Olympic quota. The double Asian Games medallist finished top in Group G in the individual event category of the qualifiers for South East Asia, Oceania. Mirza is thus in line to becoming only the third individual to have represented the country in an equestrian event at the Olympics after Imtiaz Anees (Sydney 2000) and Late Wing Commander IJ Lamba (Atlanta 1996).
Mirza finished with a total of 64 points from six qualifying events, securing 34 on his first horse Fernhill Facetime and 30 with his second horse Touchingwood.


Preparation time
30 mins to 1 hour
Cooking time
30 mins to 1 hour
Serves
Serves 6
Corned beef has been a favourite store cupboard staple for generations. Here The Hairy Bikers use it to make a simple, hearty pie.
For the filling, melt the butter with the oil in a large non-stick frying pan. Add the onion, celery, carrots and potatoes. Cook over a low heat for about 15 minutes until the vegetables are softened and beginning to colour, stirring regularly. The carrots should retain a little bite.
Add the ketchup and stir into the vegetables for a few seconds before adding the corned beef. Break the beef into chunky pieces with a wooden spoon and mix with the vegetables.
Season with pepper – you shouldn’t need salt as the corned beef is fairly salty – and remove from the heat. Leave to cool for about 20 minutes.
Preheat the oven to 190C/375F/Gas 5.
To make the pastry, put the flour, salt and butter in a food processor and pulse until the mixture resembles breadcrumbs.
Lightly whisk the egg with a tablespoon of cold water in a small bowl. Slowly pour all but one tablespoon of the egg mixture into the food processor with the motor running and blend until the mixture begins to form a ball.
Turn the pastry out onto a well-floured work surface and bring it together into a ball. Take about a third of the pastry, roll it out and use it to line a 23cm/9in pie plate or 20cm/8in round ceramic quiche dish or deep Victoria sandwich tin. Leave any excess pastry overhanging the edge. Brush the pastry edge lightly with the reserved egg.
Spoon the filling into the pastry base and spread it out evenly. Flour the surface once more and roll out the remaining pastry. Lift the rolled pastry over the rolling pin and place gently over the filling. Press the edges firmly together then trim neatly. Seal the edge with a fork.
Brush the top of the pastry with the remaining beaten egg, cut a cross in the centre of the pie and place onto a baking tray. Bake for about 40 minutes or until the pastry is golden-brown and the filling is piping hot.